ArticleAngewandte Chemie (International ed. in English)2022
Structural Insight into the Catalytic Mechanism of the Endoperoxide Synthase FtmOx1.
Article in Angewandte Chemie (International ed. in English), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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12 citing papers in PubMed, 37 citations in OpenAlex.
- Molecular Basis of α-Glycine C-H Activation by a Nonheme Fe(II)/2-Oxoglutarate Dioxygenase.Biochemistry · 2026Article
- Structural and mechanistic insights into azetidine-associated αKG-NHFe enzyme OkaE with multifunctional catalysis.Nature communications · 2026Article
- Anticancer Secondary Metabolites Produced by Fungi: Potential and Representative Compounds.International journal of molecular sciences · 2025Review
- Enzymatic Peroxidation in the Chemoenzymatic Synthesis of 13-Oxoverruculogen.Synlett : accounts and rapid communications in synthetic organic chemistry · 2025Article
- Characterizing Y224 conformational flexibility in FtmOx1-catalysis usingCatalysis science & technology · 2025Article
- Correspondence on "Structural Insight into the Catalytic Mechanism of the Endoperoxide Synthase FtmOx1".Angewandte Chemie (International ed. in English) · 2023Article
- Chemoenzymatic Synthesis of 13-Oxoverruculogen.Journal of the American Chemical Society · 2023Article
- Study and design of amino acid-based radical enzymes using unnatural amino acids.RSC chemical biology · 2023Article
- Electrospinning of n-hemin/PAN Nanocomposite Membranes and Its Photo-Enhanced Enzyme-like Catalysis.Polymers · 2022Article
- Dissecting the Mechanism of the Nonheme Iron Endoperoxidase FtmOx1 Using Substrate Analogues.JACS Au · 2022Article
- Use of Noncanonical Tyrosine Analogues to Probe Control of Radical Intermediates during Endoperoxide Installation by Verruculogen Synthase (FtmOx1).ACS catalysis · 2022Article
- Structural basis for endoperoxide-forming oxygenases.Beilstein journal of organic chemistry · 2022Review
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5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The 2-oxoglutarate (2OG)-dependent non-heme enzyme FtmOx1 catalyzes the endoperoxide biosynthesis of verruculogen. Although several mechanistic studies have been carried out, the catalytic mechanism of FtmOx1 is not well determined owing to the lack of a reliable complex structure of FtmOx1 with fumitremorgin B. Herein we provide the X-ray crystal structure of the ternary complex FtmOx1⋅2OG⋅fumitremorgin B at a resolution of 1.22 Å. Our structures show that the binding of fumitremorgin B induces significant compression of the active pocket and that Y68 is in close proximity to C26 of the substrate. Further MD simulation and QM/MM calculations support a CarC-like mechanism, in which Y68 acts as the H atom donor for quenching the C26-centered substrate radical. Our results are consistent with all available experimental data and highlight the importance of accurate complex structures in the mechanistic study of enzymatic catalysis.
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