Evidence map›Paper›PMID 34795701›Full record

ArticleAfrican health sciences2021

Phenotypic and genotypic detection of extended spectrum β-lactamases among

Souad Youssouf Kani Elmi, Medhat Saber Ashour, Fathy Zakaria Alsewy, Nashwa Fawzy Abd El Moez Azzam

Open access · diamondAbstract read
In one paragraph

Article in African health sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
0.6field-weighted citation impact, top 31% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 6 citations in OpenAlex.

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  4. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 3 institutions in 3 countries.

Souad Youssouf Kani ElmiMaster Degree in Public Health, High Institute of Public Health, Alexandria University. Egypt. Bachelor's Degree in Medical Laboratories, Faculty of Medicine and Health Sciences, Sana'a University. Yemen.
Medhat Saber AshourHigh Institute of Public Health, Alexandria University. Egypt.
Fathy Zakaria AlsewyDepartment of Diabetes and Metabolism, Faculty of Medicine, Alexandria University. Egypt.
Nashwa Fawzy Abd El Moez AzzamHigh Institute of Public Health, Alexandria University. Egypt.
Alexandria University · EGInstitute of Public Health Bengaluru · INSana'a University · YE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundT2DM patients are more likely to have UTIs caused by resistant organisms such as ESBLs producing bacteria. Challenging reliable identification and prompt characterization of in-vitro susceptibilities of these bacteria are the first steps of deciding the appropriate antimicrobial therapy for UTIs caused by them.

objectivesTo isolate and identify MATERIAL AND

methodAll samples were cultured on Cystine-Lactose-Electrolyte-Deficient Agar medium (CLED) by using calibrated loop. Growth of 100 colonies or more, i.e. 105 colony forming units (CFU)/mL urine was considered as significant bacteriuria. Isolation and identification were done according to standard method. All isolates were tested for antibiotic susceptibility testing by the disc diffusion method according to CLSI guidelines. Phenotypic detection of ESBLs was done by double-disk synergy test. Genotypic detection of blaTEM, blaSHV and blaCTX-M genes by using PCR.

resultsResults of this study showed that

conclusionblaTEM and blaSHV genes were detected in 22 out of 25 ESBLs producing

Indexed as

beta-LactamasesDiabetes Mellitus, Type 2DNA, BacterialDrug Resistance, Multiple, BacterialEscherichia coliEscherichia coli InfectionsHumansKlebsiella InfectionsKlebsiella pneumoniaePolymerase Chain ReactionUrinary Tract Infectionsbeta-LactamasesDNA, BacterialExtended Spectrum β-LactamasesPhenotypic and genotypic methodsType 2 diabetes mellitusUrinary tract infections

Identifiers

PMID34795701
PMCPMC8568203
OpenAlexW3188824322

What OpenQuestion holds

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LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.