ArticlePlant molecular biology2022
Editing of the starch branching enzyme gene SBE2 generates high-amylose storage roots in cassava.
Article in Plant molecular biology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 43 citations in OpenAlex.
- Modification of starch synthesis in food crops using CRISPR/Cas9 gene editing technology for changing climate.Stress biology · 2026Review
- Manipulation ofPlants (Basel, Switzerland) · 2025Article
- The miR395b-ABI5 module regulates amylopectin branching and biosynthesis and affects lotus root quality.Plant physiology · 2025Article
- Advancements and strategies of genetic improvement in cassava (Horticulture research · 2025Article
- The transcription factor NnNAC100 positively regulates amylopectin biosynthesis by activating NnSBEII in the rhizome of Nelumbo nucifera Gaertn.Plant cell reports · 2025Article
- Improving gene editing of CRISPR/Cas9 using the callus-specific promoterFrontiers in plant science · 2025Article
- CRISPR-Cas9 mediated editing ofFrontiers in genome editing · 2025Article
- Applications and Prospects of CRISPR/Cas9 Technology in the Breeding of Major Tropical Crops.Plants (Basel, Switzerland) · 2024Review
- An Anthocyanin-Based Visual Reporter System for Genetic Transformation and Genome Editing in Cassava.International journal of molecular sciences · 2024Article
- Cassava Breeding and Cultivation Challenges in Thailand: Past, Present, and Future Perspectives.Plants (Basel, Switzerland) · 2024Review
- Genome-wide identification and expression analysis of3 Biotech · 2023Article
- Development of cassava common mosaic virus-based vector for protein expression and gene editing in cassava.Plant methods · 2023Article
- Review
- Effects of Processing Methods and Conditioning Temperatures on the Cassava Starch Digestibility and Growth Performance of Broilers.Animals : an open access journal from MDPI · 2023Article
- CRISPR-Cas: A robust technology for enhancing consumer-preferred commercial traits in crops.Frontiers in plant science · 2023Review
- Changes in sucrose metabolism patterns affect the early maturation of Cassava sexual tetraploid roots.BMC plant biology · 2022Article
- Using the dominant mutation geneMolecular breeding : new strategies in plant improvement · 2022Article
- Screening of Induced Mutants Led to the Identification of Starch Biosynthetic Genes Associated with Improved Resistant Starch in Wheat.International journal of molecular sciences · 2022Article
- Article
- Insight into melatonin-mediated response and signaling in the regulation of plant defense under biotic stress.Plant molecular biology · 2022Review
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
key messageThe production of high-amylose cassava through CRISPR/Cas9-mediated mutagenesis of the starch branching enzyme gene SBE2 was firstly achieved. High-amylose cassava (Manihot esculenta Crantz) is desirable for starch industrial applications and production of healthier processed food for human consumption. In this study, we report the production of high-amylose cassava through CRISPR/Cas9-mediated mutagenesis of the starch branching enzyme 2 (SBE2). Mutations in two targeted exons of SBE2 were identified in all regenerated plants; these mutations, which included nucleotide insertions, and short or long deletions in the SBE2 gene, were classified into eight mutant lines. Three mutants, M6, M7 and M8, with long fragment deletions in the second exon of SBE2 showed no accumulation of SBE2 protein. After harvest from the field, significantly higher amylose (up to 56% in apparent amylose content) and resistant starch (up to 35%) was observed in these mutants compared with the wild type, leading to darker blue coloration of starch granules after quick iodine staining and altered starch viscosity with a higher pasting temperature and peak time. Further
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