ArticleThe Plant cell2022
Bypassing reproductive barriers in hybrid seeds using chemically induced epimutagenesis.
Article in The Plant cell, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 30 citations in OpenAlex.
- Cross incompatibility restricting genome exchange and diversification of primary gene pool in plants: challenges and opportunities.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026Review
- The mysterious dialogue between the embryo, endosperm, and seed coat, and its implications for seed traits.Journal of experimental botany · 2026Review
- Unraveling novel mechanisms controlling heterosis in seeds: advances and biotechnological applications in crops.Journal of experimental botany · 2025Review
- Hybridization and gene expression: Beyond differentially expressed genes.Molecular ecology · 2025Review
- Decoding hybridization barriers: the molecular and genetic orchestration of the triploid block in Arabidopsis thaliana.BMC plant biology · 2025Review
- DNA Methylation and Alternative Splicing Safeguard Genome and Transcriptome After a Retrotransposition Burst inInternational journal of molecular sciences · 2025Article
- Epigenetic gene regulation in plants and its potential applications in crop improvement.Nature reviews. Molecular cell biology · 2025Review
- Parental dialectic: Epigenetic conversations in endosperm.Current opinion in plant biology · 2024Review
- Epigenetic and transcriptional consequences in the endosperm of chemically induced transposon mobilization in Arabidopsis.Nucleic acids research · 2024Article
- Targeted suppression of siRNA biogenesis in Arabidopsis pollen promotes triploid seed viability.Nature communications · 2024Article
- Molecular basis and evolutionary drivers of endosperm-based hybridization barriers.Plant physiology · 2024Review
- Genetic Cause of Hybrid Lethality Observed in Reciprocal Interspecific Crosses betweenInternational journal of molecular sciences · 2024Article
- The Sink-Source Relationship in Cucumber (Plants (Basel, Switzerland) · 2023Article
- Role of methylation in vernalization and photoperiod pathway: a potential flowering regulator?Horticulture research · 2023Article
- Polyploidy and diploidization in soybean.Molecular breeding : new strategies in plant improvement · 2023Article
- Maternal control of triploid seed development by the TRANSPARENT TESTA 8 (TT8) transcription factor in Arabidopsis thaliana.Scientific reports · 2023Article
- Understanding and overcoming hybrid lethality in seed and seedling stages as barriers to hybridization and gene flow.Frontiers in plant science · 2023Review
- Bypassing reproductive barriers by chemical epimutagenesis.The Plant cell · 2022Article
- Triploid cultivars ofFrontiers in plant science · 2022Article
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Authors and funding
6 authors at 2 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The triploid block, which prevents interploidy hybridizations in flowering plants, is characterized by a failure in endosperm development, arrest in embryogenesis, and seed collapse. Many genetic components of triploid seed lethality have been successfully identified in the model plant Arabidopsis thaliana, most notably the paternally expressed genes (PEGs), which are upregulated in tetraploid endosperm with paternal excess. Previous studies have shown that the paternal epigenome is a key determinant of the triploid block response, as the loss of DNA methylation in diploid pollen suppresses the triploid block almost completely. Here, we demonstrate that triploid seed collapse is bypassed in Arabidopsis plants treated with the DNA methyltransferase inhibitor 5-Azacytidine during seed germination and early growth. We identified strong suppressor lines showing stable transgenerational inheritance of hypomethylation in the CG context, as well as normalized expression of PEGs in triploid seeds. Importantly, differentially methylated loci segregate in the progeny of "epimutagenized" plants, which may allow epialleles involved in the triploid block response to be identified in future studies. Finally, we demonstrate that chemically induced epimutagenesis facilitates hybridization between different Capsella species, thus potentially emerging as a strategy for producing triploids and interspecific hybrids with high agronomic interest.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.