Evidence map›Paper›PMID 34791607›Full record

ArticleEcotoxicology (London, England)2022

In vitro cultivation of primary intestinal cells from Eisenia fetida as basis for ecotoxicological studies.

Simon A B Riedl, Matthias Völkl, Anja Holzinger, Julia Jasinski, Valérie Jérôme, Thomas Scheibel, Heike Feldhaar, Ruth Freitag

Open access · hybridAbstract read
In one paragraph

Article in Ecotoxicology (London, England), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.

0numbers the graph read from it
0cells of the map it votes in
6citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

6 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
  2. Article
  3. Evaluation of the impact of polylactide microparticles on soil biota.Environmental science and pollution research international · 2025
    Article
  4. Article
  5. Article
  6. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 1 institution in 1 country.

Simon A B Riedl *Process Biotechnology, University of Bayreuth, 95440, Bayreuth, Germany.ORCID http://orcid.org/0000-0003-1699-2983
Matthias Völkl *Process Biotechnology, University of Bayreuth, 95440, Bayreuth, Germany.ORCID http://orcid.org/0000-0002-8750-8733
Anja HolzingerAnimal Ecology I, Bayreuth Center of Ecology and Environmental Research (BayCEER), University of Bayreuth, 95440, Bayreuth, Germany.ORCID http://orcid.org/0000-0002-5202-7200
Julia JasinskiBiomaterials, University of Bayreuth, 95440, Bayreuth, Germany.ORCID http://orcid.org/0000-0003-1093-5857
Valérie JérômeProcess Biotechnology, University of Bayreuth, 95440, Bayreuth, Germany.ORCID http://orcid.org/0000-0001-6492-2168
Thomas ScheibelBiomaterials, University of Bayreuth, 95440, Bayreuth, Germany.ORCID http://orcid.org/0000-0002-0457-2423
Heike FeldhaarAnimal Ecology I, Bayreuth Center of Ecology and Environmental Research (BayCEER), University of Bayreuth, 95440, Bayreuth, Germany.ORCID http://orcid.org/0000-0001-6797-5126
Ruth FreitagProcess Biotechnology, University of Bayreuth, 95440, Bayreuth, Germany. ruth.freitag@uni-bayreuth.de.ORCID http://orcid.org/0000-0002-6569-9137
University of Bayreuth · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The earthworm Eisenia fetida is a commonly used model organism for unspecific soil feeders in ecotoxicological studies. Its intestinal cells are the first to encounter possible pollutants co-ingested by the earthworm, which makes them prime candidates for studies of toxic effects of environmental pollutants on the cellular as compared to the organismic level. In this context, the aim of this study was to demonstrate the suitability of preparations of primary intestinal E. fetida cells for in vitro ecotoxicological studies. For this purpose, a suitable isolation and cultivation protocol was established. Cells were isolated directly from the intestine, maintaining >85% viability during subsequent cultivations (up to 144 h). Exposure to established pollutants and soil elutriates comprising silver nanoparticles and metal ions (Cu

Indexed as

Metal NanoparticlesOligochaetaSoil PollutantsAnimalsIntestinesPlasticsSilverSoilPlasticsSilverSoilSoil PollutantsCytotoxicityEarthwormEisenia fetidaEnvironmental pollutantsMicroplasticPrimary cells

Identifiers

PMID34791607
PMCPMC8901508
OpenAlexW3172641975

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.