ArticlePlant physiology2022
Reinforcement of CHH methylation through RNA-directed DNA methylation ensures sexual reproduction in rice.
Article in Plant physiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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29 citing papers in PubMed, 64 citations in OpenAlex.
- Mutual potentiation of IPA1 and OsNPR1 enhances rice immunity.The Plant cell · 2026Article
- Transposable element-mediated DNA methylation of the NAC20 and NAC26 promoters led to a maternal effect on grain filling.Journal of integrative plant biology · 2026Article
- Loss of function of chromatin remodeler OsCLSY4 leads to RdDM-mediated mis-expression of endosperm-specific genes affecting grain qualities.PLoS genetics · 2025Article
- The specificity landscape of WRKY transcription factors reveals the bidirectional influence of non-CG methylation.Nucleic acids research · 2025Article
- Epigenetic maps of pearl millet reveal a prominent role for CHH methylation in regulating tissue-specific gene expression.aBIOTECH · 2025Article
- DNA Methylation in Rice: Mechanisms, Regulatory Roles, and Beyond.International journal of molecular sciences · 2025Review
- An elite RNA-DEPENDENT RNA POLYMERASE3 allele enhances preharvest sprouting resistance in rice.Plant physiology · 2025Article
- Integrative Analysis of Omics Reveals RdDM Pathway Participation in the Initiation of Rice Microspore Embryogenesis Under Cold Treatment.Plants (Basel, Switzerland) · 2025Article
- Epigenetic modification brings new opportunities for gene capture by transposable elements in allopolyploidHorticulture research · 2025Article
- Identification and characterization of DICER-LIKE genes and their roles in Marchantia polymorpha development and salt stress response.The Plant journal : for cell and molecular biology · 2025Article
- Epigenetic gene regulation in plants and its potential applications in crop improvement.Nature reviews. Molecular cell biology · 2025Review
- Heterochromatin in plant meiosis.Nucleus (Austin, Tex.) · 2024Review
- Upstream regulator of genomic imprinting in rice endosperm is a small RNA-associated chromatin remodeler.Nature communications · 2024Article
- Article
- Genetic and epigenetic signatures for improved breeding of cultivated blueberry.Horticulture research · 2024Article
- Targeted suppression of siRNA biogenesis in Arabidopsis pollen promotes triploid seed viability.Nature communications · 2024Article
- Methylomes as key features for predicting recombination in some plant species.Plant molecular biology · 2024Article
- Function and regulation of plant ARGONAUTE proteins in response to environmental challenges: a review.PeerJ · 2024Review
- Variations in DNA methylation and the role of regulatory factors in rice (Frontiers in plant science · 2024Article
- DNA-dependent RNA polymerases in plants.The Plant cell · 2023Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA methylation is an important epigenetic mark that regulates the expression of genes and transposons. RNA-directed DNA methylation (RdDM) is the main molecular pathway responsible for de novo DNA methylation in plants. Although the mechanism of RdDM has been well studied in Arabidopsis (Arabidopsis thaliana), most mutations in RdDM genes cause no remarkable developmental defects in Arabidopsis. Here, we isolated and cloned Five Elements Mountain 1 (FEM1), which encodes RNA-dependent RNA polymerase 2 (OsRDR2) in rice (Oryza sativa). Mutation in OsRDR2 abolished the accumulation of 24-nt small interfering RNAs, and consequently substantially decreased genome-wide CHH (H = A, C, or T) methylation. Moreover, male and female reproductive development was disturbed, which led to sterility in osrdr2 mutants. We discovered that OsRDR2-dependent DNA methylation may regulate the expression of multiple key genes involved in stamen development, meiosis, and pollen viability. In wild-type (WT) plants but not in osrdr2 mutants, genome-wide CHH methylation levels were greater in panicles, stamens, and pistils than in seedlings. The global increase of CHH methylation in reproductive organs of the WT was mainly explained by the enhancement of RdDM activity, which includes OsRDR2 activity. Our results, which revealed a global increase in CHH methylation through enhancement of RdDM activity in reproductive organs, suggest a crucial role for OsRDR2 in the sexual reproduction of rice.
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