ArticleAnnals of translational medicine2021
Weighted gene co-expression network analysis reveals specific modules and hub genes related to immune infiltration of osteoarthritis.
Article in Annals of translational medicine, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Integrative analysis of cadmium exposure and osteoarthritis: from population evidence to molecular mechanisms.Naunyn-Schmiedeberg's archives of pharmacology · 2026Article
- Construction of a glycolysis-related diagnostic model for osteoarthritis through integrated bioinformatics analysis and machine learning.Journal of orthopaedic surgery and research · 2025Article
- Disruption of the biorhythm in gastric epithelial cell triggers inflammation in Helicobacter pylori-associated gastritis by aberrantly regulating NFIL3 via CagA activated ERK-SP1 pathway.Cell communication and signaling : CCS · 2025Article
- Integrated Bioinformatics and Experimental Validation Reveal Macrophage Polarization-Related Biomarkers for Osteoarthritis Diagnosis.Journal of multidisciplinary healthcare · 2025Article
- Identification and Validation of Fibroblast-Associated Genes in Osteoarthritis Based on High-Dimensional Weighted Gene Coexpression Network Analysis.Journal of immunology research · 2025Article
- From Genomics to Metabolomics: Molecular Insights into Osteoporosis for Enhanced Diagnostic and Therapeutic Approaches.Biomedicines · 2024Review
- Identifying Key Genes and Functionally Enriched Pathways in Osteoporotic Patients by Weighted Gene Co-Expression Network Analysis.Biochemical genetics · 2024Article
- Identification of potential pathogenic genes related to osteoporosis and osteoarthritis.Technology and health care : official journal of the European Society for Engineering and Medicine · 2024Article
- Identification of Diagnostic Markers in Synovial Tissue of Osteoarthritis by Weighted Gene Coexpression Network.Biochemical genetics · 2023Article
- Integrated bioinformatics analysis of differentially expressed genes in the temporomandibular joint internal derangement.Clinical and experimental dental research · 2023Article
- Receptor tyrosine kinase C-kit promotes a destructive phenotype of FLS in osteoarthritis via intracellular EMT signaling.Molecular medicine (Cambridge, Mass.) · 2023Article
- Identification of Key Diagnostic Markers and Immune Infiltration in Osteoarthritis.Combinatorial chemistry & high throughput screening · 2023Article
- Identification of susceptibility modules and hub genes of osteoarthritis by WGCNA analysis.Frontiers in genetics · 2022Article
- Six macrophage-associated genes in synovium constitute a novel diagnostic signature for osteoarthritis.Frontiers in immunology · 2022Article
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe incidence of osteoarthritis (OA), a chronic degenerative disease, is increasing every year. There is no effective clinical treatment for OA and the pathological mechanism remains unclear. Early diagnosis is an effective strategy to control the progress of OA. In this study, we aimed to identify potential early diagnostic biomarkers.
methodsWe downloaded the gene expression profile dataset, GSE51588 and GSE55235, from the National Center for Biotechnology Information (NCBI) Gene Expression Omnibus (GEO) public database. The differentially expressed genes (DEGs) were screened out using the "limma" R package. Weighted gene co-expression network analysis (WGCNA) was utilized to build the co-expression network between the normal and OA samples. A Venn diagram was constructed to detect the hub genes. Potential molecular mechanisms and signaling pathways were enriched by gene set variation analysis (GSVA). Single sample gene set enrichment analysis (ssGSEA) was used to identify the immune infiltration of OA.
resultsWe screened out three hub genes based on WGCNA and DEGs in this study. GSVA results showed that nuclear factor interleukin-3 (NFIL3) was related to tumor necrosis factor alpha (TNF-α) signaling via nuclear factor kappa-B (NF-κB), the reactive oxygen species pathway, and myelocytomatosis (MYC) targets v2. Highly-expressed ADM (adrenomedullin) pathways included TNF-α signaling via NF-κB, the reactive oxygen species pathway, and ultraviolet (UV) response up. OGN (osteoglycin)-enriched pathways included epithelial mesenchymal transition, coagulation, and peroxisome.
conclusionsWe identified three hub genes (
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