Evidence map›Paper›PMID 34789743›Full record

ArticleScientific reports2021

Detection of cancer stem cells by EMT-specific biomarker-based peptide ligands.

Yi-An Chen, Cheau-Ling Ho, Min-Tzu Ku, Luen Hwu, Cheng-Hsiu Lu, Sain-Jhih Chiu, Wen-Yi Chang, Ren-Shyan Liu

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
0.8field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 11 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 5 institutions in 1 country.

Yi-An ChenDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan.
Cheau-Ling HoDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan.
Min-Tzu KuDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan.
Luen HwuDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan.
Cheng-Hsiu LuCore Laboratory for Phenomics and Diagnostics, Kaohsiung Chang Gung Memorial Hospital, Kaohsiung, 833, Taiwan.
Sain-Jhih ChiuMolecular and Genetic Imaging Core/Taiwan Mouse Clinic, National Comprehensive Mouse Phenotyping and Drug Testing Center, Taipei, 112, Taiwan.
Wen-Yi ChangDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan.
Ren-Shyan LiuDepartment of Biomedical Imaging and Radiological Sciences, National Yang Ming Chiao Tung University, Taipei, 112, Taiwan. rsliu@vghtpe.gov.tw.
National Yang Ming Chiao Tung University · TWElectronics Testing Center · TWCheng Hsin General Hospital · TWKaohsiung Chang Gung Memorial Hospital · TWTaipei Veterans General Hospital · TW

Funding

Ministry of Health and Welfare MOHW 110-TDU-B-211-144019Molecular and Genetic Imaging Center, National Yang Ming Chiao Tung University and Taiwan Animal Consortium MOST- 110-2740-B-001-002
6 · The paper itself

Abstract

The occurrence of epithelial-mesenchymal transition (EMT) within tumors, which enables invasion and metastasis, is linked to cancer stem cells (CSCs) with drug and radiation resistance. We used two specific peptides, F7 and SP peptides, to detect EMT derived cells or CSCs. Human tongue squamous carcinoma cell line-SAS transfected with reporter genes was generated and followed by spheroid culture. A small molecule inhibitor-Unc0642 and low-dose ionizing radiation (IR) were used for induction of EMT. Confocal microscopic imaging and fluorescence-activated cell sorting analysis were performed to evaluate the binding ability and specificity of peptides. A SAS xenograft mouse model with EMT induction was established for assessing the binding affinity of peptides. The results showed that F7 and SP peptides not only specifically penetrated into cytoplasm of SAS cells but also bound to EMT derived cells and CSCs with high nucleolin and vimentin expression. In addition, the expression of CSC marker and the binding of peptides were increased in tumors isolated from Unc0642/IR-treated groups. Our study demonstrates the potential of these peptides for detecting EMT derived cells or CSCs and might provide an alternative isolation method for these subpopulations within the tumor in the future.

Indexed as

Epithelial-Mesenchymal TransitionAnimalsBiomarkers, TumorCarcinoma, Squamous CellCell Line, TumorCell ProliferationDimethyl SulfoxideHumansLigandsMaleMiceMice, Inbred BALB CMice, NudeNeoplastic Stem CellsPeptidesQuinazolinesBiomarkers, TumorDimethyl SulfoxideLigandsPeptidesQuinazolinesUNC0642VimentinVIM protein, human

Identifiers

PMID34789743
PMCPMC8599855
OpenAlexW3211603441

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.