Evidence map›Paper›PMID 34789179›Full record

ArticleBMC infectious diseases2021

Two extraction-free reverse transcription loop-mediated isothermal amplification assays for detection of SARS-CoV-2.

Meng Yee Lai, Fatma Diyana Mohd Bukhari, Nur Zulaikha Zulkefli, Ilyiana Ismail, Nur Izati Mustapa, Tuan Suhaila Tuan Soh, Afifah Haji Hassan, Kalaiarasu M Peariasamy, Yee Leng Lee, Jeyanthi Suppiah and 2 more

Erratum issuedOpen access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in BMC infectious diseases, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
0.2field-weighted citation impact, top 45% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 3 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 1 country.

Meng Yee LaiDepartment of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Fatma Diyana Mohd BukhariDepartment of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Nur Zulaikha ZulkefliDepartment of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia.
Ilyiana IsmailDepartment of Pathology, Hospital Sungai Buloh, Ministry of Health, Kuala Lumpur, Malaysia.
Nur Izati MustapaDepartment of Pathology, Hospital Sungai Buloh, Ministry of Health, Kuala Lumpur, Malaysia.
Tuan Suhaila Tuan SohDepartment of Pathology, Hospital Sungai Buloh, Ministry of Health, Kuala Lumpur, Malaysia.
Afifah Haji HassanDepartment of Pathology, Hospital Sungai Buloh, Ministry of Health, Kuala Lumpur, Malaysia.
Kalaiarasu M PeariasamyInstitute for Clinical Research, National Institutes of Health, Ministry of Health, Kuala Lumpur, Malaysia.
Yee Leng LeeClinical Research Centre, Hospital Sungai Buloh, Ministry of Health, Kuala Lumpur, Malaysia.
Jeyanthi SuppiahVirology Unit, Infectious Disease Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health, Kuala Lumpur, Malaysia.
Ravindran ThayanVirology Unit, Infectious Disease Research Centre, Institute for Medical Research, National Institutes of Health, Ministry of Health, Kuala Lumpur, Malaysia.
Yee Ling LauDepartment of Parasitology, Faculty of Medicine, University of Malaya, 50603, Kuala Lumpur, Malaysia. lauyeeling@um.edu.my.
Ministry of Health · MYUniversity of Malaya · MYHospital Sungai Buloh · MY

Funding

The impact of quality end-of-life care in nursing homesR01NR009547 · NINR · UNIVERSITY OF NEBRASKA MEDICAL CENTER · PI FORBES-THOMPSON, SARAH ANN · 2006 to 2010
$1.6M
NINR NIH HHS R01 NR009547
6 · The paper itself

Abstract

backgroundCurrent assays for detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) rely on time consuming, costly and laboratory based methods for virus isolation, purification and removing inhibitors. To address this limitation, we propose a simple method for testing RNA from nasopharyngeal swab samples that bypasses the RNA purification step.

methodsIn the current project, we have described two extraction-free reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for the detection of SARS-CoV-2 by using E gene and RdRp gene as the targets.

resultsHere, results showed that reverse transcription loop-mediated isothermal amplification assays with 88.4% sensitive (95% CI: 74.9-96.1%) and 67.4% sensitive (95% CI: 51.5-80.9%) for E gene and RdRp gene, respectively.

conclusionWithout the need of RNA purification, our developed RT-LAMP assays for direct detection of SARS-CoV-2 from nasopharyngeal swab samples could be turned into alternatives to qRT-PCR for rapid screening.

Indexed as

COVID-19SARS-CoV-2HumansMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesReverse TranscriptionRNA, ViralSensitivity and SpecificityRNA, ViralCoronavirusesIsothermal detectionRapid diagnosisRT-LAMPSARS-CoV-2

Identifiers

PMID34789179
PMCPMC8595270
OpenAlexW3214045536

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.