ArticleBMC genomics2021
Evaluation validation of a qPCR curve analysis method and conventional approaches.
Article in BMC genomics, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
11 citing papers in PubMed.
- Physiological Investigation of Sap-AgNPs' Cytotoxic and Gene-Modulatory Effects in Oral Squamous Cell Carcinoma.Asian Pacific journal of cancer prevention : APJCP · 2026Article
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- Pre-Amplification of Cell-Free DNA: Balancing Amplification Errors with Enhanced Sensitivity.Biomolecules · 2025Article
- A robust strategy for overexpression of DNA polymerase from Thermus aquaticus using an IPTG-independent autoinduction system in a benchtop bioreactor.Scientific reports · 2025Article
- Genome-wide identification, phylogeny, and expression analysis of PEBP gene family inFrontiers in genetics · 2025Article
- Detection of theOpen veterinary journal · 2024Article
- Introducing the fBMC bioinformatics · 2024Article
- Next-generation molecular diagnostics: Leveraging digital technologies to enhance multiplexing in real-time PCR.Trends in analytical chemistry : TRAC · 2023Article
- Evaluation validation of a qPCR curve analysis method and conventional approaches.BMC genomics · 2021Article
- Intestinal protozoa in celiac disease: Detection and phylogenetic characterization.Tropical parasitologyArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundReverse Transcription quantitative polymerase chain reaction (RT-qPCR) is a sensitive and reliable method for mRNA quantification and rapid analysis of gene expression from a large number of starting templates. It is based on the statistical significance of the beginning of exponential phase in real-time PCR kinetics, reflecting quantitative cycle of the initial target quantity and the efficiency of the PCR reaction (the fold increase of product per cycle).
resultsWe used the large clinical biomarker dataset and 94-replicates-4-dilutions set which was published previously as research tools, then proposed a new qPCR curve analysis method--C
conclusionsThe results show that C
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.