ArticleApplied and environmental microbiology2022
Selection for Phage Resistance Reduces Virulence of Shigella flexneri.
Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
25 citing papers in PubMed, 33 citations in OpenAlex.
- Bacteriophage-induced outer membrane protein remodelling drives the phenotypic switching and antibiotic re-sensitization in Klebsiella pneumoniae.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2026Article
- Giving Antibiotics a Second Chance: Evolutionary Trade-Offs and Phage-Driven Restoration of Antibiotic Susceptibility.BioDrugs : clinical immunotherapeutics, biopharmaceuticals and gene therapy · 2026Review
- An increase in environmental temperature within the growth range suppresses phage resistance inApplied and environmental microbiology · 2026Article
- Small siphophage binding to an open state of the LptDE outer membrane lipopolysaccharide translocon.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Unveiling Host Interactions and Evolutionary Constraints of a Novel Bacteriophage Infecting Xanthomonas hortorum pv. vitians.Environmental microbiology reports · 2025Article
- Rational design of a hospital-specific phage cocktail to treat Enterobacter cloacae complex infections.Nature microbiology · 2025Article
- Phage Therapy as a Novel Alternative to Antibiotics Through Adaptive Evolution and Fitness Trade-Offs.Antibiotics (Basel, Switzerland) · 2025Review
- Article
- Combating Antimicrobial Resistance: Innovative Strategies Using Peptides, Nanotechnology, Phages,Pharmaceuticals (Basel, Switzerland) · 2025Review
- Personalized inhaled bacteriophage therapy for treatment of multidrug-resistant Pseudomonas aeruginosa in cystic fibrosis.Nature medicine · 2025Article
- Oral bacteriophages and their potential as adjunctive treatments for periodontitis: a narrative review.Journal of oral microbiology · 2025Review
- Microscopic phage adsorption assay: High-throughput quantification of virus particle attachment to host bacterial cells.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Review
- Genetic characteristics and potential pathogenic agents inEmerging microbes & infections · 2024Article
- Bridging Classical Methodologies inMicroorganisms · 2024Review
- Variable fitness effects of bacteriophage resistance mutations inJournal of virology · 2024Article
- A tradeoff between bacteriophage resistance and bacterial motility is mediated by the Rcs phosphorelay inMicrobiology (Reading, England) · 2024Article
- Correlation ofInternational journal of molecular sciences · 2024Article
- Overcoming Bacteriophage Resistance in Phage Therapy.Methods in molecular biology (Clifton, N.J.) · 2024Review
- Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
There is an increasing interest in phage therapy as an alternative to antibiotics for treating bacterial infections, especially using phages that select for evolutionary trade-offs between increased phage resistance and decreased fitness traits, such as virulence, in target bacteria. A vast repertoire of virulence factors allows the opportunistic bacterial pathogen Shigella flexneri to invade human gut epithelial cells, replicate intracellularly, and evade host immunity through intercellular spread. It has been previously shown that OmpA is necessary for the intercellular spread of S. flexneri. We hypothesized that a phage which uses OmpA as a receptor to infect S. flexneri should select for phage-resistant mutants with attenuated intercellular spread. Here, we show that phage A1-1 requires OmpA as a receptor and selects for reduced virulence in S. flexneri. We characterized five phage-resistant mutants by measuring phenotypic changes in various traits: cell-membrane permeability, total lipopolysaccharide (LPS), sensitivity to antibiotics, and susceptibility to other phages. The results separated the mutants into two groups: R1 and R2 phenotypically resembled
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.