ArticleApplied and environmental microbiology2022
Characterization of Highly Ferulate-Tolerant Acinetobacter baylyi ADP1 Isolates by a Rapid Reverse Engineering Method.
Article in Applied and environmental microbiology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 28 citations in OpenAlex.
- Sustainable production of glutaric acid in microbial cell factories: Current advances and future prospects.Synthetic and systems biotechnology · 2026Review
- Characterization of carbon metabolism in a highly adhesive bacteriumFrontiers in microbiology · 2026Article
- Conversion and upgrading of syringate by Acinetobacter baylyi ADP1.Microbial cell factories · 2025Article
- Rewiring Aromatic Compound Consumption: Chromosomal Amplification and Evolution of a Foreign Pathway inACS synthetic biology · 2025Article
- Genome evolution ofApplied and environmental microbiology · 2025Article
- Cis,cis-muconic acid production from lignin related molecules byAcinetobacter baylyi ADP1.Microbial cell factories · 2025Article
- Metabolic engineering ofMetabolic engineering communications · 2024Article
- Evolution recovers the fitness of Acinetobacter baylyi strains with large deletions through mutations in deletion-specific targets and global post-transcriptional regulators.PLoS genetics · 2024Article
- Carbon-wise utilization of lignin-related compounds by synergistically employing anaerobic and aerobic bacteria.Biotechnology for biofuels and bioproducts · 2024Article
- Enhanced upgrading of lignocellulosic substrates by coculture of Saccharomyces cerevisiae and Acinetobacter baylyi ADP1.Biotechnology for biofuels and bioproducts · 2024Article
- Effects of Broad-Spectrum Antibiotic (Florfenicol) on Resistance Genes and Bacterial Community Structure of Water and Sediments in an Aquatic Microcosm Model.Antibiotics (Basel, Switzerland) · 2022Article
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adaptive laboratory evolution (ALE) is a powerful approach for improving phenotypes of microbial hosts. Evolved strains typically contain numerous mutations that can be revealed by whole-genome sequencing. However, determining the contribution of specific mutations to new phenotypes is typically challenging and laborious. This task is complicated by factors such as the mutation type, the genomic context, and the interplay between different mutations. Here, a novel approach was developed to identify the significance of mutations in strains evolved from Acinetobacter baylyi ADP1. This method, termed rapid advantageous mutation screening and selection (RAMSES), was used to analyze mutants that emerged from stepwise adaptation to and consumption of high levels of ferulate, a common lignin-derived aromatic compound. After whole-genome sequence analysis, RAMSES allowed rapid determination of effective mutations and seamless introduction of the beneficial mutations into the chromosomes of new strains with different genetic backgrounds. This simple approach to reverse engineering exploits the natural competence and high recombination efficiency of ADP1. Mutated DNA, added directly to growing cells, replaces homologous chromosomal regions to generate transformants that will become enriched if there is a selective benefit. Thus, advantageous mutations can be rapidly identified. Here, the growth advantage of transformants under selective pressure revealed key mutations in genes related to aromatic transport, including
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