ArticleMarine biotechnology (New York, N.Y.)2022
RNA-seq Analysis Reveals Alternative Splicing Under Heat Stress in Rainbow Trout (Oncorhynchus mykiss).
Article in Marine biotechnology (New York, N.Y.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers, 1 of them a synthesis that pooled it.
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Who cites it
18 citing papers in PubMed, 1 synthesis or guideline pooled it, 42 citations in OpenAlex.
- A meta-analysis of public RNA-Seq data identifies conserved stress responses in rainbow trout.BMC genomics · 2025Pooled it
- Combined Transcriptomic and Histological Profiling Uncover Hepatic Regulatory Hierarchy of TriploidBiology · 2026Article
- Molecular signatures and machine learning driven stress biomarkers for rainbow trout aquaculture and climate adaptation.Scientific reports · 2025Article
- Full-Length Transcriptome Sequencing and hsp Gene Family Analysis Provide New Insights into the Stress Response Mechanisms ofBiology · 2025Article
- Synthesis of transcriptomic studies reveals a core response to heat stress in abalone (genus Haliotis).BMC genomics · 2025Article
- Integration of mRNA and miRNA Analysis Sheds New Light on the Muscle Response to Heat Stress in Spotted Sea Bass (International journal of molecular sciences · 2024Article
- Transcriptional Rearrangements Associated with Thermal Stress and Preadaptation in Baikal Whitefish (Animals : an open access journal from MDPI · 2024Article
- Analysis of Differential Alternative Splicing in Largemouth Bass After High Temperature Exposure.Animals : an open access journal from MDPI · 2024Article
- Alternative splicing dynamically regulates common carp embryogenesis under thermal stress.BMC genomics · 2024Article
- Ribosome Profiling and RNA Sequencing Reveal Translation and Transcription Regulation under Acute Heat Stress in Rainbow Trout (International journal of molecular sciences · 2024Article
- Effects of Chronic Heat Stress on Growth, Apoptosis, Antioxidant Enzymes, Transcriptomic Profiles, and Immune-Related Genes of Hong Kong Catfish (Animals : an open access journal from MDPI · 2024Article
- Article
- Identification of Key Genes Associated with Heat Stress in Rats by Weighted Gene Co-Expression Network Analysis.Animals : an open access journal from MDPI · 2023Article
- Potential Effects of High Temperature and Heat Wave onCurrent issues in molecular biology · 2023Article
- Transcriptome Analyses Reveal Essential Roles of Alternative Splicing Regulation in Heat-Stressed Holstein Cows.International journal of molecular sciences · 2022Article
- Taxifolin Modulates Transcriptomic Response to Heat Stress in Rainbow Trout,Animals : an open access journal from MDPI · 2022Article
- The alternative splicing landscape of a coral reef fish during a marine heatwave.Ecology and evolution · 2022Article
- Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rainbow trout (Oncorhynchus mykiss) is one of the most economically important cold-water farmed species in the world, and transcriptomic studies in response to heat stress have been conducted and will be studied in depth. Alternative splicing (AS), a post-transcriptional regulatory process that regulates gene expression and increases proteomic diversity, is still poorly understood in rainbow trout under heat stress. In the present study, 18,623 alternative splicing events were identified from 9936 genes using RNA transcriptome sequencing technology (RNA-Seq) and genomic information. A total of 2731 differential alternative splicing (DAS) events were found among 2179 differentially expressed genes (DEGs). Gene ontology analysis revealed that the DEGs were mainly enriched in cellular metabolic process, cell part, and organic cyclic compound binding under heat stress. Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis displayed that the DEGs were enriched for 39 pathways, and some key pathways, such as lysine degradation, are involved in the regulation of heat stress in liver tissues of rainbow trout. The results were validated by qRT-PCR, confirming reliability of our bioinformatics analysis.
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