ArticleAmerican journal of translational research2021
Wuling San and Xiao Chaihu Decoction affect airway inflammatory response and airway smooth muscle cell proliferation in mice with allergic asthma via miR-486-5p/AQP5 axis.
Article in American journal of translational research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
2 citing papers in PubMed, 1 citations in OpenAlex.
- Distinct miRNA expression patterns in circulating eosinophil subtypes and their subtype-derived exosomes in allergic asthma.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 2026Article
- Noncoding RNAs in asthmatic airway smooth muscle cells.European respiratory review : an official journal of the European Respiratory Society · 2023Review
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectiveThis study aimed to investigate the effects of Wuling San and Xiao Chaihu Decoction on allergic asthma, and elucidate the potential mechanism of Wuling San and Xiao Chaihu Decoction for ameliorating allergic asthma.
methodsBALB/c mice were intraperitoneally injected with ovalbumin (OVA) to establish animal model of allergic asthma. Transforming growth factor beta 1 (TGF-β1) was used to induce the proliferation of airway smooth muscle cells (ASMCs) in order to establish the cell model. Quantitative real-time polymerase chain reaction (qRT-PCR) was applied to quantify the expression levels of miR-486-5p and aquaporin-5 (AQP5) in cells and tissues. Dual-luciferase reporter assay was used to verify the targeting relationship between miR-486-5p and AQP5. MTT assay and flow cytometry were carried out to evaluate cell proliferation and apoptosis, respectively. Enzyme-linked immunosorbent assay (ELISA) was conducted to measure the levels of interleukin-4 (IL-4), IL-5 and IL-13 in the bronchoalveolar lavage fluid (BALF). Hematoxylin and eosin (HE) staining and Masson staining were used to detect the recruitment of eosinophils and collagen deposition.
resultsIn both
conclusionWuling San and Xiao Chaihu Decoction can influence the proliferation and apoptosis of ASMCs and the expression of inflammatory factors in mice with allergic asthma through inhibiting the expression of miR-486-5p and upregulating the expression of AQP5.
Indexed as
Identifiers
34786062PMC8581894W3212235881What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.