ArticleNature communications2021
In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 49 citations in OpenAlex.
- Graph-based modeling of optical system enables adaptive optics on dynamic samples with self-calibration.iScience · 2026Article
- Protocol for using the multi-cellular analysis toolbox in ImageJ for single-cell calcium imaging analysis.STAR protocols · 2026Article
- Physics-informed multi-encoder adaptive optics enables rapid aberration correction for intravital microscopy of deep complex tissue.Nature communications · 2026Article
- Adaptive optical correction for in vivo two-photon fluorescence microscopy with neural fields.Nature methods · 2026Article
- High-fidelity intravital imaging of biological dynamics with latent-space-enhanced digital adaptive optics.Nature biotechnology · 2026Article
- Dual deconvolution in multiphoton structured illumination microscopy for deep-tissue super-resolution imaging.Nature communications · 2026Article
- A multicellular analysis calcium imaging toolbox for ImageJ.Cell reports methods · 2026Article
- Glutamate indicators with increased sensitivity and tailored deactivation rates.Nature methods · 2026Article
- Needle beam two-photon microscopy for simultaneous multiplane neural and vascular imaging in awake mice.PhotoniX · 2026Article
- High-throughput two-photon volumetric brain imaging in freely moving mice.Nature communications · 2025Article
- Metalens for passive generation of a droplet Bessel beam.Microsystems & nanoengineering · 2025Article
- Compact way to generate guide star for wavefront sensing in Bessel two-photon microscopy.Scientific reports · 2025Article
- Rapid axially scanned and de-scanned line-scan confocal microscopy with a tunable acoustic gradient index of refraction lens for high-speed volumetricNeurophotonics · 2025Article
- Illuminating anions in biology with genetically encoded fluorescent biosensors.Current opinion in chemical biology · 2025Review
- Modal focal adaptive optics for Bessel-focus two-photon fluorescence microscopy.Optics express · 2025Article
- Article
- Non-invasive 4D transcranial functional ultrasound and ultrasound localization microscopy for multimodal imaging of neurovascular response.Scientific reports · 2024Article
- Link Brain-Wide Projectome to Neuronal Dynamics in the Mouse Brain.Neuroscience bulletin · 2024Article
- Three-photon excited fluorescence microscopy enables imaging of blood flow, neural structure and inflammatory response deep into mouse spinal cordbioRxiv : the preprint server for biology · 2024Article
- Review
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
Studying neuronal activity at synapses requires high spatiotemporal resolution. For high spatial resolution in vivo imaging at depth, adaptive optics (AO) is required to correct sample-induced aberrations. To improve temporal resolution, Bessel focus has been combined with two-photon fluorescence microscopy (2PFM) for fast volumetric imaging at subcellular lateral resolution. To achieve both high-spatial and high-temporal resolution at depth, we develop an efficient AO method that corrects the distorted wavefront of Bessel focus at the objective focal plane and recovers diffraction-limited imaging performance. Applying AO Bessel focus scanning 2PFM to volumetric imaging of zebrafish larval and mouse brains down to 500 µm depth, we demonstrate substantial improvements in the sensitivity and resolution of structural and functional measurements of synapses in vivo. This enables volumetric measurements of synaptic calcium and glutamate activity at high accuracy, including the simultaneous recording of glutamate activity of apical and basal dendritic spines in the mouse cortex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.