ArticleNature communications2021
Directional reorientation of migrating neutrophils is limited by suppression of receptor input signaling at the cell rear through myosin II activity.
Article in Nature communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 54 citations in OpenAlex.
- Nuclear mechanobiology rules immune cells' functions: from differentiation to cell trafficking and pathogen killing.Nucleus (Austin, Tex.) · 2026Review
- Protocol for optogenetic stimulation of cells under physical confinement.STAR protocols · 2026Article
- Spontaneous membrane protrusion and cell morphogenesis via self-propelled actin filaments.EMBO reports · 2026Article
- Investigating local negative feedback of Rac activity by mathematical models and cell-motility simulations.iScience · 2026Article
- Spatial distribution of cytoskeleton-mediated feedback controls cell polarization: A computational study.PLoS computational biology · 2025Article
- Galvanin (TMEM154) is an electric-field sensor for directed cell migration.bioRxiv : the preprint server for biology · 2025Article
- Complementary cytoskeletal feedback loops control signal transduction excitability and cell polarity.Nature communications · 2025Article
- Nanoscale Curvature Regulates YAP/TAZ Nuclear Localization Through Nuclear Deformation and Rupture.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Spatial distribution of cytoskeleton-mediated feedback controls cell polarization: a computational study.bioRxiv : the preprint server for biology · 2025Article
- The Protein Kinase aPKC as Well as the Small GTPases RhoA and Cdc42 Regulates Neutrophil Chemotaxis Partly by Recruiting the ROCK Kinase to the Leading Edge.Genes to cells : devoted to molecular & cellular mechanisms · 2025Article
- Traveling-wave chemotaxis of neutrophil-like HL-60 cells.Molecular biology of the cell · 2025Article
- Follow the flow: Actin and membrane act as an integrated system to globally coordinate cell shape and movement.Current opinion in cell biology · 2024Review
- The amoeboid migration of monocytes in confining channels requires the local remodeling of the cortical actin cytoskeleton by cofilin-1.Scientific reports · 2024Article
- Principles of organelle positioning in motile and non-motile cells.EMBO reports · 2024Review
- The amoeboid migration of monocytes in confining channels requires the local remodeling of the cortical actin cytoskeleton by cofilin-1.bioRxiv : the preprint server for biology · 2024Article
- Cooperative polarization of MCAM/CD146 and ERM family proteins in melanoma.Molecular biology of the cell · 2024Article
- An Intriguing Structural Modification in Neutrophil Migration Across Blood Vessels to Inflammatory Sites: Progress in the Core Mechanisms.Cell biochemistry and biophysics · 2024Review
- Microfluidic devices for precise measurements of cell directionality reveal a role for glutamine during cell migration.Scientific reports · 2023Article
- Adaptive pathfinding by nucleokinesis during amoeboid migration.The EMBO journal · 2023Article
- A dynamic partitioning mechanism polarizes membrane protein distribution.Nature communications · 2023Article
Corrections and comments
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
To migrate efficiently to target locations, cells must integrate receptor inputs while maintaining polarity: a distinct front that leads and a rear that follows. Here we investigate what is necessary to overwrite pre-existing front-rear polarity in neutrophil-like HL60 cells migrating inside straight microfluidic channels. Using subcellular optogenetic receptor activation, we show that receptor inputs can reorient weakly polarized cells, but the rear of strongly polarized cells is refractory to new inputs. Transient stimulation reveals a multi-step repolarization process, confirming that cell rear sensitivity to receptor input is the primary determinant of large-scale directional reversal. We demonstrate that the RhoA/ROCK/myosin II pathway limits the ability of receptor inputs to signal to Cdc42 and reorient migrating neutrophils. We discover that by tuning the phosphorylation of myosin regulatory light chain we can modulate the activity and localization of myosin II and thus the amenability of the cell rear to 'listen' to receptor inputs and respond to directional reprogramming.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.