ArticleeNeuro
Off-Target Expression of Cre-Dependent Adeno-Associated Viruses in Wild-Type C57BL/6J Mice.
Article in eNeuro. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Changing subplate circuits: Early activity dependent circuit plasticity.Frontiers in cellular neuroscience · 2022Pooled it
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- WTR: A Toolkit for Functional Anterograde Transsynaptic Circuit Mapping.Research square · 2026Article
- Titer- and Intervention Timing-Dependent Functional Effects of AAV9-NeuroD1 Gene Therapy on Spinal Cord Injury.Current issues in molecular biology · 2026Article
- Neural circuits of torpor.Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology · 2026Article
- Ventral and Intermediate Hippocampus Are Required for Object-in-Place Recognition Memory in Mice.eNeuro · 2026Article
- Assessing leaky expression of Cre-dependent DNA constructs in the mouse genome using sensitive bioluminescent reporters.Cell reports methods · 2026Article
- Rational engineering of the P5 TRS-mimic site and REP78/68 start codon yields promoter variants that improve rAAV purity while maintaining high titers.Molecular therapy. Advances · 2026Article
- AAV-PHP.eB Peripheral Delivery and Central Expression in Cre Mice.The Journal of comparative neurology · 2026Article
- Differential effects of acute versus repeated chemogenetic activation of the mesocorticolimbic dopamine pathway on cocaine choice in male and female TH:Cre rats.ASPET discovery · 2026Article
- Circuit-selective pharmacological targeting of prefrontal cortex-projecting locus coeruleus neurons drives antinociception.Cell reports · 2025Article
- Exploring AAV-Mediated Gene Therapy for Inner Ear Diseases: from Preclinical Success to Clinical Potential.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- Article
- Activity of CaMKII-expressing neurons in medial prefrontal cortex of male and female Long-Evans rats is necessary for encoding odor information and novelty recognition in an odor-based incidental memory test.Cerebral cortex (New York, N.Y. : 1991) · 2025Article
- A comparison of viral strategies and model systems to target norepinephrine neurons in the locus coeruleus reveals high variability in transgene expression patterns.PLoS biology · 2025Article
- Accessing genetically defined cell types in the superior colliculus with transgenic mouse lines.iScience · 2025Article
- Tyrosine Hydroxylase-Positive Nucleus Accumbens Neurons Influence Delay Discounting in a Mouse T-Maze Task.eNeuro · 2024Article
- 5' Transgenes drive leaky expression of 3' transgenes in Cre-inducible bi-cistronic vectors.Molecular therapy. Methods & clinical development · 2024Article
- Article
- An innate immune response to adeno-associated virus genomes decreases cortical dendritic complexity and disrupts synaptic transmission.Molecular therapy : the journal of the American Society of Gene Therapy · 2024Article
Corrections and comments
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Authors and funding
6 authors.
Funding
Abstract
Adeno-associated viruses (AAVs) are a commonly used tool in neuroscience to efficiently label, trace, and/or manipulate neuronal populations. Highly specific targeting can be achieved through recombinase-dependent AAVs in combination with transgenic rodent lines that express Cre-recombinase in specific cell types. Visualization of viral expression is typically achieved through fluorescent reporter proteins (e.g., GFP or mCherry) packaged within the AAV genome. Although nonamplified fluorescence is usually sufficient to observe viral expression, immunohistochemical amplification of the fluorescent reporter is routinely used to improve viral visualization. In the present study, Cre-dependent AAVs were injected into the neocortex of wild-type C57BL/6J mice. While we observed weak but consistent nonamplified off-target double inverted open reading frame (DIO) expression in C57BL/6J mice, antibody amplification of the GFP or mCherry reporter revealed notable Cre-independent viral expression. Off-target expression of DIO constructs in wild-type C57BL/6J mice occurred independent of vendor, AAV serotype, or promoter. We also evaluated whether Cre-independent expression had functional effects via designer receptors exclusively activated by designer drugs (DREADDs). The DREADD agonist C21 (compound 21) had no effect on contextual fear conditioning or c-Fos expression in DIO-hM3Dq-mCherry
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