Evidence map›Paper›PMID 34784173›Full record

ArticleACS chemical biology2021

Visualizing an Allosteric Intermediate Using CuAAC Stabilization of an NMR Mixed Labeled Dimer.

Paul J Sapienza, Michelle M Currie, Noah M Lancaster, Kelin Li, Jeffrey Aubé, Dennis Goldfarb, Erica W Cloer, Michael B Major, Andrew L Lee

Abstract read
In one paragraph

Article in ACS chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. Article
  2. A flexible, allosteric loop regulates protein activity and rewires electrostatics.Protein science : a publication of the Protein Society · 2025
    Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Mixed, nonclassical behavior in a classic allosteric protein.Proceedings of the National Academy of Sciences of the United States of America · 2023
    Article
  8. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Paul J SapienzaDivision of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.ORCID 0000-0001-5279-3653
Michelle M CurrieDivision of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
Noah M LancasterDivision of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
Kelin LiDivision of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
Jeffrey AubéDivision of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.
Dennis GoldfarbLineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599, United States.
Erica W CloerLineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599, United States.
Michael B MajorLineberger Comprehensive Cancer Center, The University of North Carolina at Chapel Hill School of Medicine, Chapel Hill, North Carolina 27599, United States.
Andrew L LeeDivision of Chemical Biology and Medicinal Chemistry, Eshelman School of Pharmacy, The University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, United States.

Funding

Virology Research Program (Program 4)P30CA016086 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI David Darr · 1985 to 2026
$201.5M
The Role of Dynamics in Enzyme Mechanism and InhibitionR01GM083059 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI LEE, ANDREW L · 2008 to 2020
$3.7M
The Role of Protein Kinases in NRF2-driven Lung Squamous Cell CarcinomaR01CA216051 · NCI · WASHINGTON UNIVERSITY · PI MAJOR, MICHAEL BENJAMIN, WEISSMAN, BERNARD E. · 2018 to 2022
$2.5M
Structural and Dynamic Mechanisms in Classical Protein AllosteryR01GM127698 · NIGMS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI LEE, ANDREW L · 2019 to 2021
$1.2M
NCI NIH HHS P30 CA016086NCI NIH HHS R01 CA216051NIGMS NIH HHS R01 GM083059NIGMS NIH HHS R01 GM127698
6 · The paper itself

Abstract

Homodimers are the most abundant type of enzyme in cells, and as such, they represent the most elemental system for studying the phenomenon of allostery. In these systems, in which the allosteric features are manifest by the effect of the first binding event on a similar event at the second site, the most informative state is the asymmetric singly bound (lig

Indexed as

AlkynesAllosteric SiteAzidesCatalysisCopperCycloaddition ReactionDimerizationMagnetic Resonance SpectroscopyOrganometallic CompoundsProtein BindingThermodynamicsAlkynesAzidesCopperOrganometallic Compounds

Identifiers

PMID34784173
PMCPMC9141113

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.