Evidence map›Paper›PMID 34783529›Full record

ArticleAnalytical chemistry2021

Potentiometric pH Nanosensor for Intracellular Measurements: Real-Time and Continuous Assessment of Local Gradients.

Mohaddeseh Aref, Elias Ranjbari, Juan José García-Guzmán, Keke Hu, Alicia Lork, Gaston A Crespo, Andrew G Ewing, Maria Cuartero

Abstract read
In one paragraph

Article in Analytical chemistry, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Mohaddeseh ArefDepartment of Chemistry, School of Engineering Science in Chemistry, Biochemistry and Health, Royal Institute of Technology, KTH, Stockholm SE-100 44, Sweden.
Elias RanjbariDepartment of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, Gothenburg 41296, Sweden.
Juan José García-GuzmánDepartment of Chemistry, School of Engineering Science in Chemistry, Biochemistry and Health, Royal Institute of Technology, KTH, Stockholm SE-100 44, Sweden.
Keke HuDepartment of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, Gothenburg 41296, Sweden.
Alicia LorkDepartment of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, Gothenburg 41296, Sweden.
Gaston A CrespoDepartment of Chemistry, School of Engineering Science in Chemistry, Biochemistry and Health, Royal Institute of Technology, KTH, Stockholm SE-100 44, Sweden.ORCID 0000-0002-1221-3906
Andrew G EwingDepartment of Chemistry and Molecular Biology, University of Gothenburg, Kemivägen 10, Gothenburg 41296, Sweden.ORCID 0000-0002-2084-0133
Maria CuarteroDepartment of Chemistry, School of Engineering Science in Chemistry, Biochemistry and Health, Royal Institute of Technology, KTH, Stockholm SE-100 44, Sweden.ORCID 0000-0002-3858-8466

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We present a pH nanosensor conceived for single intracellular measurements. The sensing architecture consisted of a two-electrode system evaluated in the potentiometric mode. We used solid-contact carbon nanopipette electrodes tailored to produce both the indicator (pH nanosensor) and reference electrodes. The indicator electrode was a membrane-based ion-selective electrode containing a receptor for hydrogen ions that provided a favorable selectivity for intracellular measurements. The analytical features of the pH nanosensor revealed a Nernstian response (slope of -59.5 mV/pH unit) with appropriate repeatability and reproducibility (variation coefficients of <2% for the calibration parameters), a fast response time (<5 s), adequate medium-term drift (0.7 mV h

Indexed as

Ion-Selective ElectrodesProtonsHydrogen-Ion ConcentrationPotentiometryReproducibility of ResultsProtons

Identifiers

PMID34783529
PMCPMC8637545

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.