ArticleBMC veterinary research2021
Characterization and reverse genetic establishment of cattle derived Akabane virus in China.
Article in BMC veterinary research, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Review
- A neutralizing antibody protects Kunming mice against AKAV lethal challenge.BMC veterinary research · 2026Article
- Immunogenicity analyses and indirect ELISA application of a chimeric virus-like particle presenting a highly conserved peptide of Akabane virus Gc protein.Virology journal · 2025Article
- Transcriptomic analysis reveals Rnd1 to be a key player in antiviral immunity against Akabane virus via the TNF-α pathway.Archives of virology · 2025Article
- Pathogenicity analysis of a Chinese Genogroup II Akabane virus strain (TJ2016) in mouse models.Virology journal · 2025Article
- Investigating the reassortment potential and pathogenicity of the S segment in Akabane virus using a reverse genetics system.BMC veterinary research · 2025Article
- Phylogenetic analysis and biological characteristics of an Akabane virus isolated in China.Frontiers in veterinary science · 2025Article
- Isolation and identification of an AKAV strain in dairy cattle in China.Frontiers in veterinary science · 2025Article
- Development of a HiBiT-tagged reporter Akabane virus and its application in antiviral drug screening.Frontiers in cellular and infection microbiology · 2025Article
- Bioluminescent and fluorescent reporter-expressing recombinant Akabane virus (AKAV): an excellent tool to dissect viral replication.Frontiers in microbiology · 2024Article
- Generation of Stable Cell Lines Expressing Akabane Virus N Protein and Insight into Its Function in Viral Replication.Pathogens (Basel, Switzerland) · 2023Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundAkabane virus (AKAV) is an important insect-borne virus which is widely distributed throughout the world except the Europe and is considered as a great threat to herbivore health.
resultsAn AKAV strain defined as TJ2016 was firstly isolated from the bovine sera in China in 2016. Sequence analysis of the S and M segments suggested that the isolated AKAV strain was closely related to the AKAV strains JaGAr39 and JaLAB39, which belonged to AKAV genogroup II. To further study the pathogenic mechanism of AKAV, the full-length cDNA clone of TJ2016 S, M, and L segment was constructed separately into the TVT7R plasmid at the downsteam of T7 promoter and named as TVT7R-S, TVT7R-M, and TVT7R-L, respectively. The above three plasmids were further transfected into the BSR-T7/5 cells simultaneously with a ratio of 1:1:1 to produce the rescued virus AKAV. Compared with the parental wild type AKAV (wtAKAV), the rescued virus (rAKAV) was proved to be with similar cytopathic effects (CPE), plaque sizes and growth kinetics in BHK-21 cells.
conclusionWe successfully isolated a AKAV strain TJ2016 from the sera of cattle and established a reverse genetic platform for AKAV genome manipulation. The established reverse genetic system is also a powerful tool for further research on AKAV pathogenesis and even vaccine studies.
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