ArticleThe American journal of tropical medicine and hygiene2021
Diagnostic Characteristics of Lactate Dehydrogenase on a Multiplex Assay for Malaria Detection Including the Zoonotic Parasite Plasmodium knowlesi.
Article in The American journal of tropical medicine and hygiene, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 18 citations in OpenAlex.
- Strong Cross-Reactivity of Anti-Plasmodium falciparum pLDH Monoclonal Antibodies With Plasmodium malariae pLDH in the Abbott Bioline Malaria Ag P.f/P.f/P.v Rapid Diagnostic Test.Tropical medicine & international health : TM & IH · 2026Article
- Isolation and Characterisation of Phage-Displayed scFv Antibodies Targeting PfHSP70 and PfLDH ofBiochemistry research international · 2026Article
- Controlled human malaria infection studies: insights into recent advances and key immunological and ethical implementation lessons.Frontiers in immunology · 2025Review
- Analytical Sensitivity Analysis and Clinical Impact Modeling of Rapigen Rapid Diagnostic Tests for Malaria.The American journal of tropical medicine and hygiene · 2024Article
- Cloning, Expression, Purification, and Characterization of Lactate Dehydrogenase fromInternational journal of molecular sciences · 2024Article
- Comparative evaluation of the diagnostic accuracies of four different malaria rapid diagnostic test kits available in Ghana.PloS one · 2024Article
- Lactate Dehydrogenase Gene Family inAnimals : an open access journal from MDPI · 2023Article
- Comparison of two malaria multiplex immunoassays that enable quantification of malaria antigens.Malaria journal · 2022Article
- Case series of three malaria patients from Thailand infected with the simian parasite, Plasmodium cynomolgi.Malaria journal · 2022Article
- Diagnostic performance of a 5-plex malaria immunoassay in regions co-endemic for Plasmodium falciparum, P. vivax, P. knowlesi, P. malariae and P. ovale.Scientific reports · 2022Article
- Diagnostic accuracy and limit of detection of ten malaria parasite lactate dehydrogenase-based rapid tests forFrontiers in cellular and infection microbiology · 2022Article
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Authors and funding
8 authors at 2 institutions in 2 countries.
Funding
Abstract
Plasmodium lactate dehydrogenase (pLDH) is a common target in malaria rapid diagnostic tests (RDTs). These commercial antibody capture assays target either Plasmodium falciparum-specific pLDH (PfLDH), P. vivax-specific pLDH (PvLDH), or a conserved epitope in all human malaria pLDH (PanLDH). However, there are no assays specifically targeting P. ovale, P. malariae or zoonotic parasites such as P. knowlesi and P. cynomolgi. A malaria multiplex array, carrying the specific antibody spots for PfLDH, PvLDH, and PanLDH has been previously developed. This study aimed to assess potential cross-reactivity between pLDH from various Plasmodium species and this array. We tested recombinant pLDH proteins, clinical samples for P. vivax, P. falciparum, P. ovale curtisi, and P. malariae; and in vitro cultured P. knowlesi and P. cynomolgi. P. ovale-specific pLDH (PoLDH) and P. malariae-specific pLDH (PmLDH) cross-reacted with the PfLDH and PanLDH spots. Plasmodium Knowlesi-specific pLDH (PkLDH) and P. cynomolgi-specific pLDH (PcLDH) cross-reacted with the PvLDH spot, but only PkLDH was recognized by the PanLDH spot. Plasmodium ovale and P. malariae can be differentiated from P. falciparum by the concentration ratios of PanLDH/PfLDH, which had mean (range) values of 4.56 (4.07-5.16) and 4.56 (3.43-6.54), respectively, whereas P. falciparum had a lower ratio of 1.12 (0.56-2.61). Plasmodium knowlesi had a similar PanLDH/PvLDH ratio value, with P. vivax having a mean value of 2.24 (1.37-2.79). The cross-reactivity pattern of pLDH can be a useful predictor to differentiate certain Plasmodium species. Cross-reactivity of the pLDH bands in RDTs requires further investigation.
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