Evidence map›Paper›PMID 34780302›Full record

ArticleJournal of bacteriology2022

Direct RNA Nanopore Sequencing of Pseudomonas aeruginosa Clone C Transcriptomes.

Marie-Madlen Pust, Colin Francis Davenport, Lutz Wiehlmann, Burkhard Tümmler

Open access · hybridAbstract read
In one paragraph

Article in Journal of bacteriology, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.

0numbers the graph read from it
0cells of the map it votes in
17citing papers in PubMed
1.9field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

17 citing papers in PubMed, 29 citations in OpenAlex.

  1. Article
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  12. Article
  13. Detection of Streptococcus pyogenes M1Nature communications · 2023
    Article
  14. Article
  15. Article
  16. Development of ONT-cappable-seq to unravel the transcriptional landscape ofComputational and structural biotechnology journal · 2022
    Article
  17. Microbial Single-Cell Analysis: What Can We Learn From Mammalian?Frontiers in cell and developmental biology · 2021
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Marie-Madlen PustDepartment of Pediatric Pneumology, Allergology, and Neonatology, Hannover Medical School, Hannover, Germany.ORCID 0000-0001-6870-2488
Colin Francis DavenportResearch Core Unit Genomics, Hannover Medical School, Hannover, Germany.
Lutz WiehlmannResearch Core Unit Genomics, Hannover Medical School, Hannover, Germany.
Burkhard TümmlerDepartment of Pediatric Pneumology, Allergology, and Neonatology, Hannover Medical School, Hannover, Germany.ORCID 0000-0002-2566-8758
Medizinische Hochschule Hannover · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The transcriptomes of Pseudomonas aeruginosa clone C isolates NN2 and SG17M during the mid-exponential and early stationary phases of planktonic growth were evaluated by direct RNA sequencing on the nanopore platform and compared with established short-read cDNA sequencing on the Illumina platform. Fifty to ninety percent of the sense RNAs turned out to be rRNA molecules, followed by similar proportions of mRNA transcripts and noncoding RNAs. The two platforms detected similar proportions of uncharged tRNAs and 29 yet-undescribed antisense tRNAs. For example, the rarest arginine codon was paired with the most abundant tRNA

Indexed as

TranscriptomeGene Expression Regulation, BacterialGenome, BacterialGenome-Wide Association StudyNanopore SequencingPseudomonas aeruginosaRNA, BacterialRNA, Bacterialantisense RNAdirect RNA sequencingNanopore sequencingPseudomonas aeruginosatranscriptometRNA

Identifiers

PMID34780302
PMCPMC8765398
OpenAlexW3213079002

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.