Evidence map›Paper›PMID 34780047›Full record

ArticleFolia microbiologica2022

Identification of Trueperella bernardiae isolated from peking ducks (Anas platyrhynchos domesticus) by phenotypical and genotypical investigations and by a newly developed loop-mediated isothermal amplification (LAMP) assay.

Marwa F E Ahmed, Mazen Alssahen, Christoph Lämmler, Bernd Köhler, Martin Metzner, Madeleine Plötz, Amir Abdulmawjood

Open access · hybridAbstract read
In one paragraph

Article in Folia microbiologica, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 44% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 3 institutions in 2 countries.

Marwa F E AhmedHygiene and Zoonoses Department, Faculty of Veterinary Medicine, Mansoura University, Elgomhoria Street 60, Mansoura, 35516, Egypt.ORCID http://orcid.org/0000-0001-5513-0206
Mazen AlssahenInstitut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, Frankfurterstraße 85-91, 35392, Gießen, Germany.
Christoph LämmlerInstitut für Hygiene und Infektionskrankheiten der Tiere, Justus-Liebig-Universität Gießen, Frankfurterstraße 85-91, 35392, Gießen, Germany.
Bernd KöhlerRipac-Labor GmbH, Am Mühlenberg 11, 14476, Potsdam, Germany.
Martin MetznerRipac-Labor GmbH, Am Mühlenberg 11, 14476, Potsdam, Germany.
Madeleine PlötzInstitute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173, Hannover, Germany.
Amir AbdulmawjoodInstitute of Food Quality and Food Safety, University of Veterinary Medicine Hannover, Bischofsholer Damm 15, 30173, Hannover, Germany. amir.abdulmawjood@tiho-hannover.de.ORCID http://orcid.org/0000-0001-5189-8381
Justus-Liebig-Universität Gießen · DEUniversity of Veterinary Medicine Hannover, Foundation · DEMansoura University · EG

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Trueperella (T.) bernardiae is a well-known bacterial pathogen in infections of humans, rarely in animals. In the present study, five T. bernardiae isolates, isolated from five Peking ducks of four different farms, were identified by phenotypic properties, by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) analysis, and genotypically by sequencing the 16S ribosomal RNA (rRNA) gene, the superoxide dismutase A encoding gene sodA, and the glyceraldehyde-3-phosphate dehydrogenase encoding gene gap. In addition, the T. bernardiae isolates could be identified with a newly developed loop-mediated isothermal amplification (LAMP) assay based on the gyrase encoding housekeeping gene gyrA. All these tests clearly identified the T. bernardiae isolates to the species level. However, the detection of the specific gene gyrA with the newly designed LAMP assay appeared with a high sensitivity and specificity, and could help to identify this bacterial species in human and animal infections in future. The importance of the T. bernardiae isolates for the clinical condition of the ducks and for the problems at farm level remains unclear.

Indexed as

ArcanobacteriumDucksActinomycetaceaeAnimalsBeijingMolecular Diagnostic TechniquesNucleic Acid Amplification TechniquesRNA, Ribosomal, 16SSpectrometry, Mass, Matrix-Assisted Laser Desorption-IonizationRNA, Ribosomal, 16S

Identifiers

PMID34780047
PMCPMC8933347
OpenAlexW3212735090

What OpenQuestion holds

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LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.