Evidence map›Paper›PMID 34778746›Full record

ArticlePlant communications2021

Development of high-resolution multiple-SNP arrays for genetic analyses and molecular breeding through genotyping by target sequencing and liquid chip.

Zifeng Guo, Quannv Yang, Feifei Huang, Hongjian Zheng, Zhiqin Sang, Yanfen Xu, Cong Zhang, Kunsheng Wu, Jiajun Tao, Boddupalli M Prasanna and 4 more

Open access · goldAbstract readComparative Study
In one paragraph

Article in Plant communications, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 71 papers.

0numbers the graph read from it
0cells of the map it votes in
71citing papers in PubMed
19.7field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

71 citing papers in PubMed, 125 citations in OpenAlex.

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  10. Understanding and harnessing unreduced gametes for crop improvement.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Review
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  12. The Bra1K array: an efficient and versatile 1K liquid-phase SNP chip for genetics and breeding in Chinese cabbage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2026
    Article
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11 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

14 authors at 5 institutions in 3 countries.

Zifeng GuoInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Quannv YangSchool of Food Science and Engineering, Foshan University/CIMMYT-China Tropical Maize Research Center, Foshan 528225, Guangdong, China.
Feifei HuangMolBreeding Biotechnology Co., Ltd., Shijiazhuang 050035, China.
Hongjian ZhengCrop Breeding and Cultivation Research Institute, Shanghai Academy of Agricultural Sciences/CIMMYT-China Specialty Maize Research Center, Shanghai 201403, China.
Zhiqin SangXinjiang Academy of Agricultural Reclamation, Shihezi 832000, Xinjiang, China.
Yanfen XuMolBreeding Biotechnology Co., Ltd., Shijiazhuang 050035, China.
Cong ZhangMolBreeding Biotechnology Co., Ltd., Shijiazhuang 050035, China.
Kunsheng WuMolBreeding Biotechnology Co., Ltd., Shijiazhuang 050035, China.
Jiajun TaoMolBreeding Biotechnology Co., Ltd., Shijiazhuang 050035, China.
Boddupalli M PrasannaCIMMYT (International Maize and Wheat Improvement Center), ICRAF Campus, United Nations Avenue, Nairobi, Kenya.
Michael S OlsenCIMMYT (International Maize and Wheat Improvement Center), ICRAF Campus, United Nations Avenue, Nairobi, Kenya.
Yunbo WangSchool of Food Science and Engineering, Foshan University/CIMMYT-China Tropical Maize Research Center, Foshan 528225, Guangdong, China.
Jianan ZhangMolBreeding Biotechnology Co., Ltd., Shijiazhuang 050035, China.
Yunbi XuInstitute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing 100081, China.
Foshan University · CNInternational Maize and Wheat Improvement Center · KEChinese Academy of Agricultural Sciences · CNShanghai Academy of Agricultural Sciences · CNXinjiang Academy of Agricultural and Reclamation Science · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Genotyping platforms, as critical supports for genomics, genetics, and molecular breeding, have been well implemented at national institutions/universities in developed countries and multinational seed companies that possess high-throughput, automatic, large-scale, and shared facilities. In this study, we integrated an improved genotyping by target sequencing (GBTS) system with capture-in-solution (liquid chip) technology to develop a multiple single-nucleotide polymorphism (mSNP) approach in which mSNPs can be captured from a single amplicon. From one 40K maize mSNP panel, we developed three types of markers (40K mSNPs, 251K SNPs, and 690K haplotypes), and generated multiple panels with various marker densities (1K-40K mSNPs) by sequencing at different depths. Comparative genetic diversity analysis was performed with genic versus intergenic markers and di-allelic SNPs versus non-typical SNPs. Compared with the one-amplicon-one-SNP system, mSNPs and within-mSNP haplotypes are more powerful for genetic diversity detection, linkage disequilibrium decay analysis, and genome-wide association studies. The technologies, protocols, and application scenarios developed for maize in this study will serve as a model for the development of mSNP arrays and highly efficient GBTS systems in animals, plants, and microorganisms.

Indexed as

Genome, PlantGenotypeCrops, AgriculturalDNA ShufflingGenetic VariationGenome-Wide Association StudyGenotyping TechniquesOligonucleotide Array Sequence AnalysisPlant BreedingPolymorphism, Single NucleotideZea maysGBTSgenotyping by target sequencingLDlinkage disequilibriummSNPsmultiple single-nucleotide polymorphismsmultiplexing PCRsequence capture in-solution (liquid chip)

Identifiers

PMID34778746
PMCPMC8577115
OpenAlexW3187632591

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.