ArticleFrontiers in behavioral neuroscience2021
A Novel Assay Allowing Drug Self-Administration, Extinction, and Reinstatement Testing in Head-Restrained Mice.
Article in Frontiers in behavioral neuroscience, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 22 citations in OpenAlex.
- Head-fixed intravenous drug self-administration: a new frontier in addiction neuroscience.Nature protocols · 2026Article
- A Wireless Wearable Platform for Intravenous Drug Self-Administration in Freely Behaving Rats.bioRxiv : the preprint server for biology · 2026Article
- Drug self-administration in head-fixed mice.Nature protocols · 2026Review
- Goal-directed actions and habits in head-fixed mice.Frontiers in behavioral neuroscience · 2026Article
- Activation of Insula-Accumbal Projection Neurons Is Required for Relapse-Like Behaviour Following Opioid Self-Administration.Addiction biology · 2026Article
- A model of ethanol self-administration in head-fixed mice.Alcohol, clinical & experimental research · 2025Article
- Exploring the progression of drug dependence in a methamphetamine self-administration rat model through targeted and non-targeted metabolomics analyses.Scientific reports · 2024Article
- RECORD, a high-throughput, customizable system that unveils behavioral strategies leveraged by rodents during foraging-like decision-making.Communications biology · 2024Article
- Corticostriatal ensemble dynamics across heroin self-administration to reinstatement.bioRxiv : the preprint server for biology · 2024Article
- Article
- Head-mounted central venous access during optical recordings and manipulations of neural activity in mice.Nature protocols · 2024Review
- Opioidergic tuning of social attachment: reciprocal relationship between social deprivation and opioid abuse.Frontiers in neuroanatomy · 2024Review
- Review
- Article
- An Open-Source Platform for Head-Fixed Operant and Consummatory Behavior.bioRxiv : the preprint server for biology · 2023Article
- Approaches and considerations of studying neuronal ensembles: a brief review.Frontiers in cellular neuroscience · 2023Review
- An opioid-gated thalamoaccumbal circuit for the suppression of reward seeking in mice.Nature communications · 2022Article
- Two-photon calcium imaging of neuronal activity.Nature reviews. Methods primers · 2022Article
Corrections and comments
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Authors and funding
12 authors at 4 institutions in 2 countries.
Funding
Abstract
Multiphoton microscopy is one of several new technologies providing unprecedented insight into the activity dynamics and function of neural circuits. Unfortunately, some of these technologies require experimentation in head-restrained animals, limiting the behavioral repertoire that can be integrated and studied. This issue is especially evident in drug addiction research, as no laboratories have coupled multiphoton microscopy with simultaneous intravenous drug self-administration, a behavioral paradigm that has predictive validity for treatment outcomes and abuse liability. Here, we describe a new experimental assay wherein head-restrained mice will press an active lever, but not inactive lever, for intravenous delivery of heroin or cocaine. Similar to freely moving animals, we find that lever pressing is suppressed through daily extinction training and subsequently reinstated through the presentation of relapse-provoking triggers (drug-associative cues, the drug itself, and stressors). Finally, we show that head-restrained mice will show similar patterns of behavior for oral delivery of a sucrose reward, a common control used for drug self-administration experiments. Overall, these data demonstrate the feasibility of combining drug self-administration experiments with technologies that require head-restraint, such as multiphoton imaging. The assay described could be replicated by interested labs with readily available materials to aid in identifying the neural underpinnings of substance use disorder.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.