Evidence map›Paper›PMID 34776737›Full record

ArticleBreeding science2021

Sat-BSA: an NGS-based method using local

Tenta Segawa, Chisato Nishiyama, Muluneh Tamiru-Oli, Yu Sugihara, Akira Abe, Hinako Sone, Noriaki Itoh, Mayu Asukai, Aiko Uemura, Kaori Oikawa and 6 more

Open access · diamondAbstract read
In one paragraph

Article in Breeding science, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed, 1 pooled it
1.8field-weighted citation impact, top 14% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 1 synthesis or guideline pooled it, 10 citations in OpenAlex.

  1. Pooled it
  2. Article
  3. Cutting edge tools in the field of soil microbiology.Current research in microbial sciences · 2024
    Review
  4. Non-vernalization requirement for flowering in Brassica rapa conferred by a dominant allele of FLOWERING LOCUS T.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2023
    Article
  5. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

16 authors at 4 institutions in 2 countries.

Tenta SegawaIshikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Chisato NishiyamaIshikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Muluneh Tamiru-OliDepartment of Animal, Plant and Soil Sciences, AgriBio Building, La Trobe University, 5 Ring Road, Bundoora, VIC 3086, Australia.
Yu SugiharaKyoto University, Nakajou 1, Mozume, Mukou, Kyoto 617-0001, Japan.
Akira AbeIwate Biotechnology Research Center, 22-174-4, Narita, Kitakami, Iwate 024-0003, Japan.
Hinako SoneIshikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Noriaki ItohIshikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Mayu AsukaiIshikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Aiko UemuraIwate Biotechnology Research Center, 22-174-4, Narita, Kitakami, Iwate 024-0003, Japan.
Kaori OikawaIwate Biotechnology Research Center, 22-174-4, Narita, Kitakami, Iwate 024-0003, Japan.
Hiroe UtsushiIwate Biotechnology Research Center, 22-174-4, Narita, Kitakami, Iwate 024-0003, Japan.
Ayako Ikegami-KatayamaIshikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Tomohiro ImamuraIshikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Masashi MoriIshikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Ryohei TerauchiKyoto University, Nakajou 1, Mozume, Mukou, Kyoto 617-0001, Japan.
Hiroki TakagiIshikawa Prefectural University, 1-308, Suematsu, Nonoichi, Ishikawa 921-8836, Japan.
Ishikawa Prefectural University · JPIwate Biotechnology Research Center · JPKyoto University · JPAgriBio · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Advances in next generation sequencing (NGS)-based methodologies have accelerated the identifications of simple genetic variants such as point mutations and small insertions/deletions (InDels). Structural variants (SVs) including large InDels and rearrangements provide vital sources of genetic diversity for plant breeding. However, their analysis remains a challenge due to their complex nature. Consequently, novel NGS-based approaches are needed to rapidly and accurately identify SVs. Here, we present an NGS-based bulked-segregant analysis (BSA) technique called Sat-BSA (SVs associated with traits) for identifying SVs controlling traits of interest in crops. Sat-BSA targets allele frequencies at all SNP positions to first identify candidate genomic regions associated with a trait, which is then reconstructed by long reads-based local

Indexed as

Brassica rapabulked-segregant analysisde novo assemblynext-generation sequencingOxford Nanopore sequencingstructural variants

Identifiers

PMID34776737
PMCPMC8573553
OpenAlexW3170500541

What OpenQuestion holds

Textmetadata
LicenceCC BY-NC-ND
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.