Evidence map›Paper›PMID 34776223›Full record

ArticleMethods in enzymology2021

Visualizing replication fork encounters with DNA interstrand crosslinks.

Ryan C James, Marina A Bellani, Jing Zhang, Jing Huang, Althaf Shaik, Durga Pokharel, Himabindu Gali, Julia Gichimu, Arun K Thazhathveetil, Michael M Seidman

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Methods in enzymology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 77% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors at 8 institutions in 2 countries.

Ryan C JamesDepartment of Molecular Biology and Genetics, Cornell University, Ithaca, NY, United States.
Marina A BellaniLaboratory of Molecular Biology and Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD, United States.
Jing ZhangDepartment of Neurosurgery, Shanghai East Hospital, Tongji University School of Medicine, Shanghai, China.
Jing HuangInstitute of Chemical Biology and Nanomedicine, State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Biology, Hunan University, Changsha, China.
Althaf ShaikLaboratory of Molecular Biology and Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD, United States.
Durga PokharelHorizon Discovery, Lafayette, CO, United States.
Himabindu GaliFrederick National Laboratory for Cancer Research, Frederick, MD, United States.
Julia GichimuLaboratory of Molecular Biology and Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD, United States.
Arun K ThazhathveetilNitto Denko Avecia Inc., Cincinnati, OH, United States.
Michael M SeidmanLaboratory of Molecular Biology and Immunology, National Institute on Aging, National Institutes of Health, Baltimore, MD, United States. Electronic address: seidmanm@grc.nia.nih.gov.
National Institutes of Health · USCornell University · USFrederick National Laboratory for Cancer Research · USHorizon Discovery Group (United States) · USNational Institute on Aging · USNitto (United States) · USShanghai East Hospital · CNState Key Laboratory of Chemobiosensing and Chemometrics

Funding

Repair of localized DNA damageZ01AG000746 · NIA · NATIONAL INSTITUTE ON AGING · PI SEIDMAN, MICHAEL · 2007 to 2008
$607k
Intramural NIH HHS Z01 AG000746
6 · The paper itself

Abstract

Replication forks encounter numerous challenges as they move through eu- and hetero-chromatin during S phase in mammalian cells. These include a variety of impediments to the unwinding of DNA by the replicative helicase such as alternate DNA structures, transcription complexes and R-loops, DNA-protein complexes, and DNA chemical adducts. Much of our knowledge of these events is based on analysis of markers of the replication stress and DNA Damage Response that follow stalling of replisomes. To examine consequences for the replisomes more directly, we developed an approach for imaging collisions of replication forks with the potent block presented by an interstrand crosslink (ICL). The strategy is based on the visualization on DNA fibers of the encounter of replication tracts and an antigen tagged ICL. Our studies revealed an unexpected restart of DNA synthesis past an intact ICL. In addition, and also unexpected, we found two distinct versions of the replisome, one biased toward euchromatin and the other more prominent in heterochromatin. Here, we present details of our experimental procedures that led to these observations.

Indexed as

DNA HelicasesDNA ReplicationAnimalsDNADNA-Binding ProteinsDNA DamageDNA RepairMammalsDNADNA-Binding ProteinsDNA HelicasesDNA fiberDONSONFANCMInterstrand crosslinkReplication stressReplication traverse

Identifiers

PMID34776223
PMCPMC10035509
OpenAlexW3214675207

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.