ArticleJournal of clinical virology : the official publication of the Pan American Society for Clinical Virology2021
Safe and effective pool testing for SARS-CoV-2 detection.
Article in Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Efficient high-risk human papillomavirus screening through pooled testing: a simulation study based on real-world data in low- and middle-income countries.Journal of global health · 2026Article
- Effect of sample vortex mixing on severe acute respiratory syndrome coronavirus 2 nucleic acid testing: Comparative analysis of individual samples and sample pools.The Journal of international medical research · 2025Article
- Aggregating multiple test results to improve medical decision-making.PLoS computational biology · 2025Article
- Flexible upscaling of laboratory PCR testing capacity at the Robert Koch Institute during the SARS-CoV-2 pandemic.Virology journal · 2023Article
- SARS-CoV-2 PCR Positivity in Elementary School Classes in Bavaria in the School Year 2021/22—Initial Results From the Evaluation of the Bavarian Pool Test Project in Elementary and Special Schools.Deutsches Arzteblatt international · 2022Article
- Effective high-throughput RT-qPCR screening for SARS-CoV-2 infections in children.Nature communications · 2022Article
- Mobile PCR-based surveillance for SARS-CoV-2 to reduce visiting restrictions in nursing homes during the COVID-19 pandemic: a pilot study.Infection · 2022Article
- Determining the reliability of rapid SARS-CoV-2 antigen detection in fully vaccinated individuals.Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2022Article
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- Pooled RT-qPCR testing for SARS-CoV-2 surveillance in schools - a cluster randomised trial.EClinicalMedicine · 2021Article
- Pooling for SARS-CoV2 Surveillance: Validation and Strategy for Implementation in K-12 Schools.Frontiers in public health · 2021Article
Corrections and comments
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Authors and funding
18 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
objectivesThe global spread of SARS-CoV-2 is a serious public health issue. Large-scale surveillance screenings are crucial but can exceed test capacities. We (A) optimized test conditions and (B) implemented pool testing of respiratory swabs into SARS-CoV-2 diagnostics. STUDY
design(A) We determined the optimal pooling strategy and pool size. In addition, we measured the impact of vortexing prior to sample processing, compared a pipette-pooling method (by combining transport medium of several specimens) and a swab-pooling method (by combining several swabs into a test tube filled with PBS) as well as determined the sensitivities of three PCR assays. (B) Finally, we applied high-throughput pool testing for diagnostics.
results(A) In a low prevalence setting, we defined a preferable pool size of ten in a two-stage hierarchical pool testing strategy. Vortexing of swabs (n = 33) increased cellular yield by a factor of 2.34. By comparing Ct-values of 16 pools generated with two different pooling strategies, pipette-pooling was more efficient compared to swab-pooling. Measuring dilution series of 20 SARS-CoV-2 positive samples in three PCR assays simultaneously revealed detection rates of 85% (assay I), 50% (assay II), and 95% (assay III) at a 1:100 dilution. (B) We systematically pooled 55,690 samples in a period of 44 weeks resulting in a reduction of 47,369 PCR reactions.
conclusionsFor implementing pooling strategies into high-throughput diagnostics, we recommend utilizing a pipette-pooling method, performing sensitivity validation of the PCR assays used, and vortexing swabs prior to analyses. Pool testing for SARS-CoV-2 detection is feasible and effective in a low prevalence setting.
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