Evidence map›Paper›PMID 34773179›Full record

ArticleMolecular biology reports2022

Quercetin and HSC70 coregulate the anti-inflammatory action of the ubiquitin-like protein MNSFβ.

Morihiko Nakamura, Yuki Fukuma, Kaori Notsu, Megumi Kono

Abstract read
PubMed Publisher
In one paragraph

Article in Molecular biology reports, 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
0.6field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 7 citations in OpenAlex.

  1. Review
  2. Quercetin: A Potential Polydynamic Drug.Molecules (Basel, Switzerland) · 2023
    Review
  3. Review
  4. Pharmacological Activity of Quercetin: An Updated Review.Evidence-based complementary and alternative medicine : eCAM · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Morihiko NakamuraThe Department of Cooperative Medical Research, Office for Regional Collaboration and Innovation, Shimane University, Izumo, 693-8501, Japan. nkmr0515@med.shimane-u.ac.jp.ORCID http://orcid.org/0000-0003-1079-9787
Yuki FukumaThe Department of Cooperative Medical Research, Office for Regional Collaboration and Innovation, Shimane University, Izumo, 693-8501, Japan.
Kaori NotsuThe Department of Cooperative Medical Research, Office for Regional Collaboration and Innovation, Shimane University, Izumo, 693-8501, Japan.
Megumi KonoThe Department of Cooperative Medical Research, Office for Regional Collaboration and Innovation, Shimane University, Izumo, 693-8501, Japan.
Shimane University · JP

Funding

Grant-in-Aid for Scientific Research, from the Ministry of Education, Culture, Sports, Science and Technology of Japan 17K07335
6 · The paper itself

Abstract

backgroundQuercetin is a flavonol that modifies many cellular processes. Monoclonal nonspecific suppressor factor β is a member of the ubiquitin-like family of proteins that are involved in various biological processes. It has been demonstrated that quercetin regulates the effect of MNSFβ on tumor necrosis factor-α secretion in lipopolysaccharide (LPS)-stimulated macrophages. This study found that quercetin and the heat shock protein HSC70 coregulate the action of MNSFβ. METHODS AND

resultsQuercetin dose-dependently suppressed the LPS/interferon γ-induced nitric oxide production without cytotoxicity in the macrophage-like cell line Raw264.7. SiRNA knockdown experiments showed that quercetin inhibited the MNSFβ and HSC70 siRNA-mediated enhancement of TNFα and the production of RANTES, a member of C-C chemokine superfamily, in LPS-stimulated Raw264.7 cells. Western blot analysis showed that quercetin and HSC70 regulated ERK1/2 activation and LPS-stimulated IκBα degradation by affecting the complex formation of MNSFβ and the proapoptotic protein Bcl-G. Moreover, MNSFβ is implicated in TLR4/MyD88 signaling but not in TLR3 signaling.

conclusionsHSC70 is an important chaperone that facilitates the stabilization of MNSFβ. Quercetin may negatively control the function of MNSFβ by regulating the action of the molecular chaperone HSC70. MNSFβ mediates TLR4/Myd88 signaling but not TLR3 signaling.

Indexed as

AnimalsCell LineFlavonoidsHSC70 Heat-Shock ProteinsInterferon-gammaLipopolysaccharidesMacrophagesMiceNitric OxideQuercetinRAW 264.7 CellsSignal TransductionSuppressor Factors, ImmunologicTumor Necrosis Factor-alphaUbiquitinUbiquitins3-hydroxyflavoneFlavonoidsHSC70 Heat-Shock ProteinsInterferon-gammaLipopolysaccharidesmonoclonal nonspecific suppressor factorNitric OxideQuercetinSuppressor Factors, ImmunologicTumor Necrosis Factor-alphaUbiquitinUbiquitinsMolecular chaperone HSC70QuercetinUbiquitin-like protein MNSFβ

Identifiers

PMID34773179
OpenAlexW3213003809

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.