ArticleJournal of animal science and biotechnology2021
Metabolomic fingerprinting of pig seminal plasma identifies in vivo fertility biomarkers.
Article in Journal of animal science and biotechnology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed, 22 citations in OpenAlex.
- Seminal Extracellular Vesicles as Molecular Modulators of Male Reproductive Function.Reproduction in domestic animals = Zuchthygiene · 2026Review
- Biomarkers of sperm fertility in pigs: a relevant tool for the discrimination of sub‑fertile males?Animal reproduction · 2026Article
- L-Glutamine Supplementation Improves the In Vitro Qualitative Parameters of Cryopreserved Qinchuan Bull Sperm.Animals : an open access journal from MDPI · 2025Article
- Comprehensive Integrated Analyses of Proteins and Metabolites in Equine Seminal Plasma (Horses and Donkeys).Proteomes · 2025Article
- Nuclear Magnetic Resonance (NMR) Metabolomics: Current Applications in Equine Health Assessment.Metabolites · 2024Review
- Impact of inclusion of post-spermatic ejaculate fraction in boar seminal doses on sperm metabolism, quality, and interaction with uterine fluid.Scientific reports · 2023Article
- Comparison of Growth Performance and Plasma Metabolomics between Two Sire-Breeds of Pigs in China.Genes · 2023Article
- Sperm physiology and in vitro fertilising ability rely on basal metabolic activity: insights from the pig model.Communications biology · 2023Article
- Exploring the metabolome of seminal plasma in two different horse types: Light versus draft stallions.Reproduction in domestic animals = Zuchthygiene · 2023Article
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Authors and funding
8 authors at 4 institutions in 2 countries.
Funding
Abstract
backgroundMetabolomic approaches, which include the study of low molecular weight molecules, are an emerging -omics technology useful for identification of biomarkers. In this field, nuclear magnetic resonance (NMR) spectroscopy has already been used to uncover (in) fertility biomarkers in the seminal plasma (SP) of several mammalian species. However, NMR studies profiling the porcine SP metabolome to uncover in vivo fertility biomarkers are yet to be carried out. Thus, this study aimed to evaluate the putative relationship between SP-metabolites and in vivo fertility outcomes. To this end, 24 entire ejaculates (three ejaculates per boar) were collected from artificial insemination (AI)-boars throughout a year (one ejaculate every 4 months). Immediately after collection, ejaculates were centrifuged to obtain SP-samples, which were stored for subsequent metabolomic analysis by NMR spectroscopy. Fertility outcomes from 1525 inseminations were recorded over a year, including farrowing rate, litter size, stillbirths per litter and the duration of pregnancy.
resultsA total of 24 metabolites were identified and quantified in all SP-samples. Receiver operating characteristic (ROC) curve analysis showed that lactate levels in SP had discriminative capacity for farrowing rate (area under the curve [AUC] = 0.764) while carnitine (AUC = 0.847), hypotaurine (AUC = 0.819), sn-glycero-3-phosphocholine (AUC = 0.833), glutamate (AUC = 0.799) and glucose (AUC = 0.750) showed it for litter size. Similarly, citrate (AUC = 0.743), creatine (AUC = 0.812), phenylalanine (AUC = 0.750), tyrosine (AUC = 0.753) and malonate (AUC = 0.868) levels had discriminative capacity for stillbirths per litter; and malonate (AUC = 0.767) and fumarate (AUC = 0.868) levels for gestation length.
conclusionsThe assessment of selected SP-metabolites in ejaculates through NMR spectroscopy could be considered as a promising non-invasive tool to predict in vivo fertility outcomes in pigs. Moreover, supplementing AI-doses with specific metabolites should also be envisaged as a way to improve their fertility potential.
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