Evidence map›Paper›PMID 34772403›Full record

ArticleCancer cell international2021

Nestin is essential for cellular redox homeostasis and gastric cancer metastasis through the mediation of the Keap1-Nrf2 axis.

Jing Lv, Meiqiang Xie, Shufen Zhao, Wensheng Qiu, Shasha Wang, Manming Cao

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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3 · Its place in the literature

Who cites it

14 citing papers in PubMed.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Review
  6. Article
  7. Review
  8. Review
  9. Distribution, contribution and regulation of nestinJournal of advanced research · 2024
    Review
  10. Energy metabolism: a new target for gastric cancer treatment.Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico · 2024
    Review
  11. Article
  12. Review
  13. Review
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Jing Lv *Department of Oncology, The Affiliated Hospital of Qingdao University/Key Laboratory of Cancer Molecular and Translational Research, The Affiliated Hospital of Qingdao University, No.16 Jiangsu Road, Qingdao, 266000, Shandong, China.
Meiqiang Xie *Medical Oncology, Central People's Hospital of Zhanjiang, Zhanjiang, 524000, Guangdong, China.
Shufen ZhaoDepartment of Oncology, The Affiliated Hospital of Qingdao University/Key Laboratory of Cancer Molecular and Translational Research, The Affiliated Hospital of Qingdao University, No.16 Jiangsu Road, Qingdao, 266000, Shandong, China.
Wensheng QiuDepartment of Oncology, The Affiliated Hospital of Qingdao University/Key Laboratory of Cancer Molecular and Translational Research, The Affiliated Hospital of Qingdao University, No.16 Jiangsu Road, Qingdao, 266000, Shandong, China.
Shasha WangDepartment of Oncology, The Affiliated Hospital of Qingdao University/Key Laboratory of Cancer Molecular and Translational Research, The Affiliated Hospital of Qingdao University, No.16 Jiangsu Road, Qingdao, 266000, Shandong, China. wss_0528@126.com.
Manming CaoDepartment of Medical Oncology, Zhujiang Hospital, Southern Medical University, No.253 Gongye Road, Haizhu District, Guangzhou, 510282, Guangdong, China. caomanming@smu.edu.cn.ORCID http://orcid.org/0000-0003-1805-0922

Funding

National Natural Science Foundation of China 81402579the Key Research and Development Foundation Projects of Shandong Province 2017GSF218109
6 · The paper itself

Abstract

backgroundGastric cancer (GC) is a common malignancy of the digestive system. Antioxidant activity is regarded as a possible mechanism in ectopic cancer. Hence, oxidative stress regulation is being evaluated for cancer treatment. Previous research has demonstrated that Nestin is associated with antioxidative resistance via its modulation of the Kelch-like ECH-associated protein 1 (Keap1)-nuclear factor erythroid 2-related factor 2 (Nrf2) pathway.

methodsWe determined the role of Nestin-mediated redox homeostasis and tumor phenotypes in GC cells.

resultsWe found that the Nestin expression level was high in GC tissues and cell lines. Nestin knockdown in the GC cell lines SGC-7901 and MKN-45 reduced viability, induced apoptosis, decreased antioxidant enzyme generation, and repressed GC metastasis. Nestin binds to Keap1, resulting in Nrf2 degradation and influencing downstream gene expression. Nestin knockdown resulted in the downregulation of Nrf2 expression in GC cells. The restoration of Nrf2 expression or treatment with the Nrf2 activator sulforaphane counteracted the inhibitory effect of Nestin knockdown on the proliferation, migration, invasion, and antioxidant enzyme production in GC cells. Moreover, xenograft GC tumors exhibited a slower growth rate than those of the control group in vivo.

conclusionsTaken together, these findings suggest that the Nestin-Keap1-Nrf2 axis confers oxidative stress resistance and plays an important role in the proliferation, migration, and invasion of GC cells.

Indexed as

ApoptosisGastric cancerKeap1MetastasisNestinNrf2Oxidative stress

Identifiers

PMID34772403
PMCPMC8588700

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