Evidence map›Paper›PMID 34771781›Full record

ArticleMaterials (Basel, Switzerland)2021

In Vitro Evaluation of Acellular Collagen Matrices Derived from Porcine Pericardium: Influence of the Sterilization Method on Its Biological Properties.

Rone Aparecido De Grandis, Larissa Natiele Miotto, Luis Eduardo Genaro, Larissa Migliatti Polli, Ana Maria de Guzzi Plepis, Fabiana Tessari Rodrigues, Virginia da Conceição Amaro Martins, Leonardo Pereira Franchi, Raquel Mantuaneli Scarel-Caminaga, Ticiana Sidorenko de Oliveira Capote

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Materials (Basel, Switzerland), 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Article
  2. Review
  3. Article
  4. [Research advances of natural biomaterials in promoting wound repair].Zhonghua shao shang yu chuang mian xiu fu za zhi · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Rone Aparecido De GrandisFaculty of Pharmaceutical Sciences-FCFar, Paulista State University, Araraquara 14801-903, SP, Brazil.
Larissa Natiele MiottoDepartment of Morphology, Genetics, Orthodontic and Pediatric Dentistry, Araraquara School of Dentistry, Paulista State University, Araraquara 14801-903, SP, Brazil.
Luis Eduardo GenaroDepartment of Morphology, Genetics, Orthodontic and Pediatric Dentistry, Araraquara School of Dentistry, Paulista State University, Araraquara 14801-903, SP, Brazil.
Larissa Migliatti PolliFaculty of Pharmaceutical Sciences-FCFar, Paulista State University, Araraquara 14801-903, SP, Brazil.
Ana Maria de Guzzi PlepisDepartment of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos, University of São Paulo, Sao Carlos 13566-590, SP, Brazil.
Fabiana Tessari RodriguesDepartment of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos, University of São Paulo, Sao Carlos 13566-590, SP, Brazil.
Virginia da Conceição Amaro MartinsDepartment of Chemistry and Molecular Physics, Institute of Chemistry of São Carlos, University of São Paulo, Sao Carlos 13566-590, SP, Brazil.
Leonardo Pereira FranchiDepartment of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Sao Carlos 13566-590, SP, Brazil.
Raquel Mantuaneli Scarel-CaminagaDepartment of Morphology, Genetics, Orthodontic and Pediatric Dentistry, Araraquara School of Dentistry, Paulista State University, Araraquara 14801-903, SP, Brazil.
Ticiana Sidorenko de Oliveira CapoteDepartment of Morphology, Genetics, Orthodontic and Pediatric Dentistry, Araraquara School of Dentistry, Paulista State University, Araraquara 14801-903, SP, Brazil.

Funding

Fundação de Amparo à Pesquisa do Estado de São Paulo 2011/17723-0
6 · The paper itself

Abstract

The aim of this study were characterize acellular collagen matrices derived from porcine pericardium (PP) and to evaluate their properties after sterilization by ethylene oxide and gamma ray. PP matrices were subjected to alkaline hydrolysis (AH), and samples were characterized for biological stability, membrane thickness measurements, differential scanning calorimetry (DSC) and scanning electron microscopy (SEM). Subsequently, the matrices were frozen, lyophilized and sterilized by ethylene oxide or gamma radiation. For in vitro assays, CHO-K1 cell culture was used and evaluated for cytotoxicity, clonogenic survival assay, genotoxicity and mutagenicity. Analysis of variance (ANOVA) was used, followed by Dunnett's post-test, with a significance level of 5%. After AH, there was no significant change in matrix thickness. The relative biodegradability of the material after implantation was observed. Morphology and dimensions had small changes after AH. As for cell viability, none of the tested matrices showed a statistically significant difference (

Indexed as

collagen matricesporcine pericardiumtissue regeneration

Identifiers

PMID34771781
PMCPMC8584648

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.