Evidence map›Paper›PMID 34769691›Full record

ArticleInternational journal of environmental research and public health2021

Evaluation of Residual Infectivity after SARS-CoV-2 Aerosol Transmission in a Controlled Laboratory Setting.

Luisa Zupin, Sabina Licen, Margherita Milani, Libera Clemente, Lorenzo Martello, Sabrina Semeraro, Francesco Fontana, Maurizio Ruscio, Alessandro Miani, Sergio Crovella and 1 more

Abstract read
In one paragraph

Article in International journal of environmental research and public health, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.

0numbers the graph read from it
0cells of the map it votes in
8citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

8 citing papers in PubMed.

  1. A Virus Aerosol Chamber Study: The Impact of UVA, UVC, and HEnvironment & health (Washington, D.C.) · 2025
    Article
  2. Article
  3. Review
  4. Article
  5. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Luisa ZupinInstitute for Maternal and Child Health, IRCCS Burlo Garofolo, Via dell'Istria 65/1, 34137 Trieste, Italy.ORCID 0000-0001-5886-9129
Sabina LicenDepartment of Chemical and Pharmaceutical Sciences, University of Trieste, Via L. Giorgieri 1, 34127 Trieste, Italy.ORCID 0000-0002-8895-396X
Margherita MilaniDepartment of Medicine, Surgery and Health Sciences, University of Trieste, Strada di Fiume 447, 34137 Trieste, Italy.
Libera ClementeOspedale San Polo, Azienda Sanitaria Universitaria Giuliano Isontina, Via Luigi Galvani 1, 34074 Monfalcone, Italy.ORCID 0000-0002-2264-6967
Lorenzo MartelloDepartment of Chemical and Pharmaceutical Sciences, University of Trieste, Via L. Giorgieri 1, 34127 Trieste, Italy.ORCID 0000-0003-3614-4906
Sabrina SemeraroINSTM National Interuniversity Consortium of Materials Science and Technology, Via G. Giusti 9, 50121 Firenze, Italy.ORCID 0000-0002-3707-9117
Francesco FontanaOspedale San Polo, Azienda Sanitaria Universitaria Giuliano Isontina, Via Luigi Galvani 1, 34074 Monfalcone, Italy.
Maurizio RuscioOspedale Maggiore, Azienda Sanitaria Universitaria Giuliano Isontina, Piazza dell'Ospitale 1, 34129 Trieste, Italy.ORCID 0000-0002-3446-6613
Alessandro MianiDepartment of Environmental Science and Policy, University of Milan, Via Festa del Perdono 7, 20122 Milano, Italy.
Sergio CrovellaDepartment of Biological and Environmental Sciences, College of Arts and Sciences, University of Qatar, Doha 2713, Qatar.ORCID 0000-0001-8493-1168
Pierluigi BarbieriDepartment of Chemical and Pharmaceutical Sciences, University of Trieste, Via L. Giorgieri 1, 34127 Trieste, Italy.

Funding

IRCCS Burlo Garofolo /Italian Ministry of Health RC 15/17, 03/20, 47/20University of Trieste Microgrants 2021
6 · The paper itself

Abstract

Severe acute respiratory syndrome coronavirus type 2 (SARS-CoV-2) is mainly transmitted through respiratory droplets, aerosols, or direct contact with fomites from an infected subject. It has been reported that SARS-CoV-2 is stable and viable in aerosol up to 16 h in controlled laboratory conditions. However, the aerosolization conditions varied a lot between the studies. In this work, an experimental laboratory model of SARS-CoV-2 aerosolization was established, employing an impinger nebulizer, a cylindrical chamber for aerosol travel, and a SKC biosampler for the collection of particles. The efficiency of the system was assessed based on the molecular determination of the viral load in the nebulizer after the aerosolization and in the aerosol collected at the end of the travel. Moreover, the residual infectivity was tested in vitro on the Vero E6 cell line, through the observation of the cytopathic effect (CPE), and the quantification of the viral load in the supernatants at 7 days post inoculation (dpi). A high RNA viral load was found in the SKC biosampler after aerosolization, indicating that it was possible to transport a high virus titer through the 30-cm chamber with all the dilutions (initial 10

Indexed as

COVID-19SARS-CoV-2AerosolsFomitesHumansLaboratoriesAerosolsaerosolresidual infectivitySARS-CoV-2

Identifiers

PMID34769691
PMCPMC8582643

What OpenQuestion holds

Textmetadata
LicenceCC BY
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.