Evidence map›Paper›PMID 34769421›Full record

ArticleInternational journal of molecular sciences2021

Structural Analysis of the Ancestral Haloalkane Dehalogenase AncLinB-DmbA.

Andrii Mazur, Pavel Grinkevich, Radka Chaloupkova, Petra Havlickova, Barbora Kascakova, Michal Kuty, Jiri Damborsky, Ivana Kuta Smatanova, Tatyana Prudnikova

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact, top 88% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 2 institutions in 1 country.

Andrii MazurFaculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.ORCID 0000-0002-4318-6568
Pavel GrinkevichFaculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.ORCID 0000-0003-1523-7708
Radka ChaloupkovaLoschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.
Petra HavlickovaFaculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.
Barbora KascakovaFaculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.
Michal KutyFaculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.
Jiri DamborskyLoschmidt Laboratories, Department of Experimental Biology and RECETOX, Faculty of Science, Masaryk University, Kamenice 5, 625 00 Brno, Czech Republic.ORCID 0000-0002-7848-8216
Ivana Kuta SmatanovaFaculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.ORCID 0000-0002-1337-9481
Tatyana PrudnikovaFaculty of Science, University of South Bohemia in Ceske Budejovice, Branisovska 1760, 370 05 Ceske Budejovice, Czech Republic.ORCID 0000-0002-9969-1993
University of South Bohemia in České Budějovice · CZMasaryk University · CZ

Funding

Czech Ministry of Education CZ.02.1.01/0.0/0.0/16_026/0008451Czech Ministry of Education LM2018121ERDF CZ.02.1.01/0.0/0.0/15_003/0000441GAJU 17/2019/P
6 · The paper itself

Abstract

Haloalkane dehalogenases (EC 3.8.1.5) play an important role in hydrolytic degradation of halogenated compounds, resulting in a halide ion, a proton, and an alcohol. They are used in biocatalysis, bioremediation, and biosensing of environmental pollutants and also for molecular tagging in cell biology. The method of ancestral sequence reconstruction leads to prediction of sequences of ancestral enzymes allowing their experimental characterization. Based on the sequences of modern haloalkane dehalogenases from the subfamily II, the most common ancestor of thoroughly characterized enzymes LinB from

Indexed as

Binding SitesCatalytic DomainCrystallography, X-RayEvolution, MolecularHydrolasesHydrolysisModels, MolecularMycobacterium bovisProtein EngineeringSequence Analysis, ProteinSphingomonadaceaehaloalkane dehalogenaseHydrolasesancestral sequence reconstructionhaloalkane dehalogenasehalogenated pollutantsstructural analysis

Identifiers

PMID34769421
PMCPMC8584953
OpenAlexW3212486690

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.