ArticleInternational journal of molecular sciences2021
Recombinase Polymerase Amplification Assay with and without Nuclease-Dependent-Labeled Oligonucleotide Probe.
Article in International journal of molecular sciences, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed, 18 citations in OpenAlex.
- Article
- Isothermal Nucleic Acid Amplification-Based Lateral Flow Testing for the Detection of Plant Viruses.International journal of molecular sciences · 2024Review
- CRISPR/Cas12a-based method coupled with isothermal amplification to identifyFrontiers in microbiology · 2024Article
- Development of a novel integrated isothermal amplification system for detection of bacteria-spiked blood samples.AMB Express · 2023Article
- Comparison of Biosensing Methods Based on Different Isothermal Amplification Strategies: A Case Study withBiosensors · 2022Article
- Rapid On-Site Detection of theFrontiers in plant science · 2022Article
- A versatile integrated tube for rapid and visual SARS-CoV-2 detection.Frontiers in microbiology · 2022Article
- Recent advances in recombinase polymerase amplification: Principle, advantages, disadvantages and applications.Frontiers in cellular and infection microbiology · 2022Review
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The combination of recombinase polymerase amplification (RPA) and lateral flow test (LFT) is a strong diagnostic tool for rapid pathogen detection in resource-limited conditions. Here, we compared two methods generating labeled RPA amplicons following their detection by LFT: (1) the basic one with primers modified with different tags at the terminals and (2) the nuclease-dependent one with the primers and labeled oligonucleotide probe for nuclease digestion that was recommended for the high specificity of the assay. Using both methods, we developed an RPA-LFT assay for the detection of worldwide distributed phytopathogen-alfalfa mosaic virus (AMV). A forward primer modified with fluorescein and a reverse primer with biotin and fluorescein-labeled oligonucleotide probe were designed and verified by RPA. Both labeling approaches and their related assays were characterized using the in vitro-transcribed mRNA of AMV and reverse transcription reaction. The results demonstrated that the RPA-LFT assay based on primers-labeling detected 10
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Registered trials
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