ArticleThe journal of physical chemistry. B2021
Single-Molecule Localization Microscopy of 3D Orientation and Anisotropic Wobble Using a Polarized Vortex Point Spread Function.
Article in The journal of physical chemistry. B, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Optimal excitation polarization schemes to boost the precision of single-molecule orientation imaging.Biomedical optics express · 2026Article
- Single Fluorogens and Orientation-Localization Microscopy for Quantifying Chemical and Biomolecular Dynamics at the Nanoscale.Accounts of chemical research · 2026Article
- Efficient spatio-angular reconstruction enables high-fidelity mapping of six-dimensional structures and dynamics with polarized fluorescence microscopy.Nature communications · 2026Article
- Fused deep learning enables 6D single-molecule localization in polarization-resolved microscopy.Methods and applications in fluorescence · 2026Article
- 4polar3D single molecule imaging of 3D orientation in dense actin networks using ratiometric polarization splitting.Nature communications · 2026Article
- Mapping Optical Chirality with Single Fluorescent Molecules.Nano letters · 2026Article
- Single-shot Stokes polarimetry of plasmon-coupled single-molecule fluorescence.Nanophotonics (Berlin, Germany) · 2025Article
- Single molecule orientation and localization microscopy.Nature photonics · 2025Article
- Resolution in super-resolution microscopy - facts, artifacts, technological advancements and biological applications.Journal of cell science · 2025Article
- Differential interactions determine anisotropies at interfaces of RNA-based biomolecular condensates.Nature communications · 2025Article
- Resolving the Orientations of and Angular Separation Between a Pair of Dipole Emitters.Physical review letters · 2025Article
- Single-molecule orientation-localization microscopy: Applications and approaches.Quarterly reviews of biophysics · 2024Review
- POLCAM: instant molecular orientation microscopy for the life sciences.Nature methods · 2024Article
- Fundamental Limits in Measuring the Anisotropic Rotational Diffusion of Single Molecules.The journal of physical chemistry. A · 2024Article
- Single-Molecule Orientation Imaging Reveals the Nano-Architecture of Amyloid Fibrils Undergoing Growth and Decay.Nano letters · 2024Article
- Single-Molecule Orientation Imaging Reveals the Nano-Architecture of Amyloid Fibrils Undergoing Growth and Decay.bioRxiv : the preprint server for biology · 2024Article
- Resolving the Nanoscale Structure of β-Sheet Peptide Self-Assemblies Using Single-Molecule Orientation-Localization Microscopy.ACS nano · 2024Article
- From Molecules to Classrooms: A Comprehensive Guide to Single-Molecule Localization Microscopy.Journal of chemical education · 2024Article
- Getting the Most Out of Fluorogenic Probes: Challenges and Opportunities in Using Single-Molecule Fluorescence to Image Electro- and Photocatalysis.Chemical & biomedical imaging · 2023Review
- Extracellular matrix sensing via modulation of orientational order of integrins and F-actin in focal adhesions.Life science alliance · 2023Article
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Authors and funding
2 authors.
Funding
Abstract
Within condensed matter, single fluorophores are sensitive probes of their chemical environments, but it is difficult to use their limited photon budget to image precisely their positions, 3D orientations, and rotational diffusion simultaneously. We demonstrate the polarized vortex point spread function (PSF) for measuring these parameters, including characterizing the anisotropy of a molecule's wobble, simultaneously from a single image. Even when imaging dim emitters (∼500 photons detected), the polarized vortex PSF can obtain 12 nm localization precision, 4°-8° orientation precision, and 26° wobble precision. We use the vortex PSF to measure the emission anisotropy of fluorescent beads, the wobble dynamics of Nile red (NR) within supported lipid bilayers, and the distinct orientation signatures of NR in contact with amyloid-beta fibrils, oligomers, and tangles. The unparalleled sensitivity of the vortex PSF transforms single-molecule microscopes into nanoscale orientation imaging spectrometers, where the orientations and wobbles of individual probes reveal structures and organization of soft matter that are nearly impossible to perceive by using molecular positions alone.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.