Evidence map›Paper›PMID 34766309›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

Evaluating Efficacy of Peptide-Delivered Oligonucleotides Using the Severe Taiwanese SMA Mouse Model.

Larissa Goli, Jessica Stoodley, Suzan M Hammond, Richard Raz

Abstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
5.0field-weighted citation impact, top 4% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

  1. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors at 1 institution in 1 country.

Larissa Goli *Department of Paediatrics, University of Oxford, Oxford, UK.
Jessica Stoodley *Department of Paediatrics, University of Oxford, Oxford, UK.
Suzan M HammondDepartment of Paediatrics, University of Oxford, Oxford, UK.
Richard RazDepartment of Paediatrics, University of Oxford, Oxford, UK. Richard.Raz@paediatrics.ox.ac.uk.
University of Oxford · GB

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Oligonucleotides (ONs) are therapeutic macromolecules with great potential for the treatment of neurological conditions, including spinal muscular atrophy (SMA), a neurodegenerative disease. However, the neurovascular unit severely limits their distribution to the neural parenchyma of the brain and the spinal cord. Cell-penetrating peptides (CPPs) can be conjugated to oligonucleotides to increase their delivery across biological barriers. In this chapter, we describe the synthesis and conjugation of CPPs to oligonucleotides, and the use of a severe SMA mouse model to test in vivo the efficacy of CPP-delivered oligonucleotides, using ELISA, western blot, and TaqMan™ RT-qPCR assays.

Indexed as

Muscular Atrophy, SpinalAnimalsCell-Penetrating PeptidesDisease Models, AnimalMiceOligonucleotidesOligonucleotides, AntisenseCell-Penetrating PeptidesOligonucleotidesOligonucleotides, AntisenseAntisense oligonucleotidesCell-penetrating peptideIn vivo pup administrationsPeptide oligonucleotide conjugatesSpinal muscular atrophySurvival motor neuronTaiwanese SMA mouse model

Identifiers

PMID34766309
OpenAlexW3214045132

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.