Evidence map›Paper›PMID 34766264›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2022

High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry.

Marco A Fernandez, Hammad Alzayat, Maria C Jaimes, Yacine Kharraz, Gerard Requena, Pedro Mendez

Erratum issuedAbstract read
PubMed Publisher
In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2022. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
3.7field-weighted citation impact, top 6% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 9 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Development of Spectral Imaging Cytometry.Methods in molecular biology (Clifton, N.J.) · 2023
    Review
4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Marco A FernandezFlow Cytometry Facility, Germans Trias i Pujol Research Institute, Badalona, Spain.
Hammad AlzayatFlow Cytometry Facility, Germans Trias i Pujol Research Institute, Badalona, Spain.
Maria C JaimesCytek Biosciences, Fremont, CA, USA.
Yacine KharrazCytek Biosciences, Fremont, CA, USA.
Gerard RequenaFlow Cytometry Facility, Germans Trias i Pujol Research Institute, Badalona, Spain.
Pedro MendezSynthego Corporation, Menlo Park, CA, USA. pmendez00@gmail.com.
Institut d'Investigació en Ciències de la Salut Germans Trias i Pujol · ESSynthego (United States) · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A comprehensive study of the cellular components of the immune system demands both deep and broad immunophenotyping of numerous cell subsets in an effective and practical way. Novel full-spectrum technology reveals the complete emission spectrum of each dye maximizing the amount of information that can be obtained on a single sample regarding conventional flow cytometry and provide an expanded knowledge of biological processes. In this chapter, we describe a 37-color protocol that allows to identify more than 45 different cell populations on whole blood samples of SARS-CoV-2-infected patients.

Indexed as

COVID-19Flow CytometryColorHumansImmune SystemImmunophenotypingDeep immunophenotypingFull-spectrum cytometrySpectral signature

Identifiers

PMID34766264
OpenAlexW3211949298

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.