ArticleCell reports methods2021
SeqStain is an efficient method for multiplexed, spatialomic profiling of human and murine tissues.
Article in Cell reports methods, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Emerging technologies in the spatial proteomics landscape.Analytical and bioanalytical chemistry · 2026Review
- Antibody-oligonucleotide conjugates for spatial proteomics: principles, applications, and challenges.Acta biochimica et biophysica Sinica · 2025Article
- Single-cell spatial proteomics.Histology and histopathology · 2025Review
- Article
- Super-resolved highly multiplexed immunofluorescence imaging for precise protein localization and podocyte ultrastructure.Journal of cellular and molecular medicine · 2024Article
- A novel dual-release scaffold for fluorescent labels improves cyclic immunofluorescence.RSC chemical biology · 2024Article
- Quantitative performance assessment of Ultivue multiplex panels in formalin-fixed, paraffin-embedded human and murine tumor specimens.Scientific reports · 2024Article
- Fully automated sequential immunofluorescence (seqIF) for hyperplex spatial proteomics.Scientific reports · 2023Article
- Multiplexed Single-Cell in Situ Protein Profiling.ACS measurement science au · 2022Review
- Spatial mapping of protein composition and tissue organization: a primer for multiplexed antibody-based imaging.Nature methods · 2022Review
- Automated multimodal fluorescence microscopy for hyperplex spatial-proteomics: Coupling microfluidic-based immunofluorescence to high resolution, high sensitivity, three-dimensional analysis of histological slides.Frontiers in oncology · 2022Article
- Next-Generation Pathology Using Multiplexed Immunohistochemistry: Mapping Tissue Architecture at Single-Cell Level.Frontiers in oncology · 2022Review
- Multiplexed imaging and automated signal quantification in formalin-fixed paraffin-embedded tissues by ChipCytometry.Cell reports methods · 2021Article
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8 authors.
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Abstract
Spatial organization of molecules and cells in complex tissue microenvironments provides essential organizational cues in health and disease. A significant need exists for improved visualization of these spatial relationships. Here, we describe a multiplex immunofluorescence imaging method, termed SeqStain, that uses fluorescent-DNA-labeled antibodies for immunofluorescent staining and nuclease treatment for de-staining that allows selective enzymatic removal of the fluorescent signal. SeqStain can be used with primary antibodies, secondary antibodies, and antibody fragments to efficiently analyze complex cells and tissues. Additionally, incorporation of specific endonuclease restriction sites in antibody labels allows for selective removal of fluorescent signals while retaining other signals that can serve as marks for subsequent analyses. The application of SeqStain on human kidney tissue provided a spatialomic profile of the organization of >25 markers in the kidney, highlighting it as a versatile, easy-to-use, and gentle new technique for spatialomic analyses of complex microenvironments.
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