ArticleSTAR protocols2021
A low-cost and open-source protocol to produce key enzymes for molecular detection assays.
Article in STAR protocols, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- RT-RPA-Assisted CRISPR/Cas12a-Based Isothermal Detection of Chikungunya Virus.Methods in molecular biology (Clifton, N.J.) · 2027Article
- Versatile and portable Cas12a-mediated detection of antibiotic resistance markers.Scientific reports · 2026Article
- LAMP-coupled CRISPR-Cas12a assays: A promising new tool for molecular diagnosis of leishmaniasis.PLoS neglected tropical diseases · 2026Article
- Thermal optimized PCR coupled to CRISPR-Cas12a for rapid detection of blaOXA-1 resistance gene.PloS one · 2026Article
- Versatile and Portable Cas12a-mediated Detection of Antibiotic Resistance Markers.bioRxiv : the preprint server for biology · 2025Article
- Article
- Open access methods and protocols promote open science in a pandemic.STAR protocols · 2022Article
- Novel CRISPR-based detection ofFrontiers in microbiology · 2022Article
- UnCovid: A versatile, low-cost, and open-source protocol for SARS-CoV-2 RNA detection.STAR protocols · 2021Article
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10 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Here, we describe a detailed step-by-step protocol for the expression, purification, quantification, and activity determination of key enzymes for molecular detection of pathogens. Based on previous reports, we optimized the protocol for LbCas12a, Taq DNA polymerase, M-MLV reverse transcriptase, and TEV protease to make it compatible with minimal laboratory equipment, broadly available in low- and middle-income countries. The enzymes produced with this protocol have been successfully used for molecular detection applications. For complete details on the use and execution of this protocol, please refer to Alcántara et al. (2021a, 2021b).
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Registered trials
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