ArticleFrontiers in cell and developmental biology2021
Capacitation-Induced Mitochondrial Activity Is Required for Sperm Fertilizing Ability in Mice by Modulating Hyperactivation.
Article in Frontiers in cell and developmental biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Regulation of mitochondrial function during spermatogenesis and sperm maturation.Cellular & molecular biology letters · 2026Review
- High-Resolution Imaging Reveals Mitochondrial Protein Imbalance in Sperm of Oligoasthenospermic Men.Molecular reproduction and development · 2026Article
- Sperm subpopulations differing in mitochondrial abundance show divergent nuclear allele frequencies.PloS one · 2026Article
- Glucose and pyruvate differentially modulate metabolic and redox dynamics during capacitation to enable fertilization competence in mouse sperm.Reproductive biology and endocrinology : RB&E · 2025Article
- Beyond swimming: emerging parameters for predicting the fertility of mouse spermatozoa.Lab animal · 2025Review
- Nitrogen-Doped Carbon Quantum Dots Enhance In Vitro Fertilization and Early Embryo Development by Improving Sperm Activation in Mice.Development & reproduction · 2025Article
- Effects of Embryo Production Method and Culture Medium on Embryonic Development in Red-Rumped Agouti.Cell biology international · 2025Article
- Evaluating DAPI stain to assess sperm membrane integrity by flow cytometry in livestock species.Veterinary research communications · 2025Article
- Precursor A-Kinase Anchor Protein 4 as a Predictive Biomarker of Post-Thaw Semen Quality in Goats.Veterinary sciences · 2025Article
- Lactobacillus rhamnosus PB01 Oral Supplementation Alleviates Metabolic and Reproductive Dysfunctions in Type 1 Diabetic Male Rats.Molecular nutrition & food research · 2025Article
- Capacitation of ram spermatozoa promotes changes in energy metabolism and aquaporin 3 and is affected by individual testosterone variations.Andrology · 2025Article
- Article
- Effect of Capacitation on Proteomic Profile and Mitochondrial Parameters of Spermatozoa in Bulls.Journal of proteome research · 2025Article
- Effects of a homozygous missense mutation in theZhong nan da xue xue bao. Yi xue ban = Journal of Central South University. Medical sciences · 2025Article
- A side-by-side comparison of different capacitation media in developing mouse sperm fertilizing ability.Scientific reports · 2024Article
- Uncommon opsin's retinal isomer is involved in mammalian sperm thermotaxis.Scientific reports · 2024Article
- Embryo development is impaired by sperm mitochondrial-derived ROS.Biological research · 2024Article
- Article
- Membrane potential hyperpolarization: a critical factor in acrosomal exocytosis and fertilization in sperm within the female reproductive tract.Frontiers in cell and developmental biology · 2024Article
- Bull Sperm SWATH-MS-Based Proteomics Reveals Link between High Fertility and Energy Production, Motility Structures, and Sperm-Oocyte Interaction.Journal of proteome research · 2023Article
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7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
To become fully competent to fertilize an egg, mammalian sperm undergo a series of functional changes within the female tract, known as capacitation, that require an adequate supply and management of energy. However, the contribution of each ATP generating pathway to sustain the capacitation-associated changes remains unclear. Based on this, we investigated the role of mitochondrial activity in the acquisition of sperm fertilizing ability during capacitation in mice. For this purpose, the dynamics of the mitochondrial membrane potential (MMP) was studied by flow cytometry with the probe tetramethylrhodamine ethyl ester (TMRE). We observed a time-dependent increase in MMP only in capacitated sperm as well as a specific staining with the probe in the flagellar region where mitochondria are confined. The MMP rise was prevented when sperm were exposed to the mitochondrial uncoupler carbonyl cyanide m-chlorophenyl hydrazine (CCCP) or the protein kinase A (PKA) inhibitor H89 during capacitation, indicating that MMP increase is dependent on capacitation and H89-sensitive events. Results showed that whereas nearly all motile sperm were TMRE positive, immotile cells were mostly TMRE negative, supporting an association between high MMP and sperm motility. Furthermore, CCCP treatment during capacitation did not affect PKA substrate and tyrosine phosphorylations but produced a decrease in hyperactivation measured by computer assisted sperm analysis (CASA), similar to that observed after H89 exposure. In addition, CCCP inhibited the
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