Evidence map›Paper›PMID 34764473›Full record

ArticleNature chemical biology2021

Single-molecule imaging of glycan-lectin interactions on cells with Glyco-PAINT.

Roger Riera, Tim P Hogervorst, Ward Doelman, Yan Ni, Silvia Pujals, Evangelia Bolli, Jeroen D C Codée, Sander I van Kasteren, Lorenzo Albertazzi

Registry-linked trialAbstract read
PubMed Publisher
In one paragraph

Article in Nature chemical biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT06105177 (Implementation of Up-front ctDNA Analysis Into Lung Cancer Care and Development of Liquid Biopsy-based Decision Support Models - the Lungmarker² Study), which is not on this map. Cited by 28 papers.

0numbers the graph read from it
0cells of the map it votes in
28citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

NCT06105177 recruitingnot on this mapstarted 2023, after this paper: background citation

Implementation of Up-front ctDNA Analysis Into Lung Cancer Care and Development of Liquid Biopsy-based Decision Support Models - the Lungmarker² Study

Typeobservational_patient_registrySponsorCatharina Ziekenhuis EindhovenRan2023 to 2026Enrolled800ConditionsLung Cancer
3 · Its place in the literature

Who cites it

28 citing papers in PubMed.

  1. Review
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Review
  15. Article
  16. CryoSeek II: Cryo-EM analysis of glycofibrils from freshwater reveals well-structured glycans coating linear tetrapeptide repeats.Proceedings of the National Academy of Sciences of the United States of America · 2025
    Article
  17. Article
  18. Visualizing the Heterogeneity in Homogeneous Supramolecular Polymers.Journal of the American Chemical Society · 2024
    Article
  19. Article
  20. Beyond DNA: new probes for PAINT super-resolution microscopy.Chemical communications (Cambridge, England) · 2023
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Roger Riera *Department of Biomedical Engineering, Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands.
Tim P Hogervorst *Department of Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands.
Ward DoelmanDepartment of Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands.ORCID http://orcid.org/0000-0002-5138-9402
Yan NiLaboratory of Chemical Biology, Department of Biomedical Engineering and Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands.ORCID http://orcid.org/0000-0001-8409-0477
Silvia PujalsNanoscopy for Nanomedicine, Institute for Bioengineering of Catalonia, Barcelona, Spain.
Evangelia BolliCellular and Molecular Immunology, Bioengineering Sciences Department, Vrije Universiteit Brussel, Brussels, Belgium.
Jeroen D C CodéeDepartment of Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands.ORCID http://orcid.org/0000-0003-3531-2138
Sander I van KasterenDepartment of Bio-Organic Synthesis, Leiden Institute of Chemistry, Leiden University, Leiden, the Netherlands. s.i.van.kasteren@chem.leidenuniv.nl.ORCID http://orcid.org/0000-0003-3733-818X
Lorenzo AlbertazziDepartment of Biomedical Engineering, Institute for Complex Molecular Systems, Eindhoven University of Technology, Eindhoven, the Netherlands. l.albertazzi@tue.nl.ORCID http://orcid.org/0000-0002-6837-0812

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Most lectins bind carbohydrate ligands with relatively low affinity, making the identification of optimal ligands challenging. Here we introduce a point accumulation in nanoscale topography (PAINT) super-resolution microscopy method to capture weak glycan-lectin interactions at the single-molecule level in living cells (Glyco-PAINT). Glyco-PAINT exploits weak and reversible sugar binding to directly achieve single-molecule detection and quantification in cells and is used to establish the relative k

Indexed as

AnimalsCell MembraneCell Membrane PermeabilityCHO CellsCricetulusKineticsLectinsLigandsMultivariate AnalysisPolysaccharidesProtein BindingSingle Molecule ImagingSmall Molecule LibrariesStructure-Activity RelationshipLectinsLigandsPolysaccharidesSmall Molecule Libraries

Identifiers

What OpenQuestion holds

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Registered trials

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.