ArticleScientific reports2021
Rapid characterization of feline leukemia virus infective stages by a novel nested recombinase polymerase amplification (RPA) and reverse transcriptase-RPA.
Article in Scientific reports, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 21 citations in OpenAlex.
- Clinical and virological effects of the integrase inhibitor raltegravir in cats with naturally progressive feline leukaemia virus infection.Journal of feline medicine and surgery · 2026Article
- A newly developed multiplex RT-PCR enables detection of blood-associated viral nucleic acids in cats.Veterinary research communications · 2026Article
- Innovative Colorimetric Neutral Red-Based Loop-Mediated Isothermal Amplification (NR-LAMP) Assay: Transforming Rapid and Affordable Feline Leukemia Virus Detection.International journal of molecular sciences · 2025Article
- PCR testing of conjunctival swabs to detect feline leukaemia virus in domestic cats in Thailand.The veterinary quarterly · 2025Article
- Longitudinal observation of chronic domestic cat hepadnavirus infection in cats with evidence of extrahepatic involvement.Journal of feline medicine and surgery · 2025Article
- Development of Reverse Transcriptase Recombinase Polymerase Amplification Combined with Lateral Flow Dipstick for Rapid Detection of Tilapia Lake Virus (TiLV): Pilot Study.Veterinary sciences · 2025Article
- Pathological alteration and caspase-dependent activity of feline kidneys in natural infection of feline morbillivirus.BMC veterinary research · 2025Article
- Advances in Simple, Rapid, and Contamination-Free Instantaneous Nucleic Acid Devices for Pathogen Detection.Biosensors · 2023Review
- Discordant FeLV p27 immunoassay and PCR test results in 21 cats with hematologic disorders.Journal of feline medicine and surgery · 2023Article
- RT-nestRPA is a new technology for the rapid and sensitive detection of nucleic acid detection of pathogens used for a variety of medical application scenarios.Analytica chimica acta · 2023Article
- Lateral Flow Recombinase Polymerase Amplification Assays for the Detection of Human Plasmodium Species.The American journal of tropical medicine and hygiene · 2023Article
- Diagnostic utility of LDH measurement for determining the etiology of modified transudate pleural effusion in cats.Frontiers in veterinary science · 2022Article
- Salivary peptidomic profiling of chronic gingivostomatitis in cats by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and nanoscale liquid chromatography-tandem mass spectrometry.Journal of veterinary internal medicineArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Feline leukemia virus (FeLV) is a major viral disease in cats, causing leukemia and lymphoma. The molecular detection of FeLV RNA and the DNA provirus are important for staging of the disease. However, the rapid immunochromatographic assay commonly used for antigen detection can only detect viremia at the progressive stage. In this study, nested recombinase polymerase amplification (nRPA) was developed for exogenous FeLV DNA provirus detection, and reverse transcriptase polymerase amplification (RT-RPA) was developed for the detection of FeLV RNA. The approaches were validated using 108 cats with clinicopathologic abnormalities due to FeLV infection, and from 14 healthy cats in a vaccination plan. The nRPA and RT-RPA assays could rapidly amplify the FeLV template, and produced high sensitivity and specificity. The FeLV detection rate in regression cats by nRPA was increased up to 45.8% compared to the rapid immunochromatographic assay. Hence, FeLV diagnosis using nRPA and RT-RPA are rapid and easily established in low resource settings, benefiting FeLV prognosis, prevention, and control of both horizontal and vertical transmission.
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