ArticleJBMR plus2021
Controlled Formation of Carboxymethyllysine in Bone Matrix through Designed Glycation Reaction.
Article in JBMR plus, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
21 citing papers in PubMed, 28 citations in OpenAlex.
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- Bone Organoids: A Novel Tool for Modeling and Managing Skeletal Disorders in Diabetes.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Upregulation of Parathyroid Hormone Receptor 1 (International journal of molecular sciences · 2026Article
- Glycoxidation of the bone matrix modulates mineralization.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2025Article
- Fecal microbiota transplantation in mice improves bone material properties through altered mineral quality.JBMR plus · 2025Article
- F2-Isoprostanes Are Associated With Increased Fracture Risk in Type 2 Diabetes.The Journal of clinical endocrinology and metabolism · 2025Observational
- Article
- Long-duration type 1 diabetes is associated with deficient cortical bone mechanical behavior and altered matrix composition in human femoral bone.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2024Article
- Review
- Review
- Role of Advanced Glycation End-Products and Oxidative Stress in Type-2-Diabetes-Induced Bone Fragility and Implications on Fracture Risk Stratification.Antioxidants (Basel, Switzerland) · 2023Review
- Structural role of osteocalcin and its modification in bone fracture.Applied physics reviews · 2023Article
- Skeletal manifestations in a streptozotocin-induced C57BL/6 model of Type 1 diabetes.Bone reports · 2022Article
- Collagen cross-link profiles and mineral are different between the mandible and femur with site specific response to perturbed collagen.Bone reports · 2022Article
- Degradation of Bone Quality in a Transgenic Mouse Model of Alzheimer's Disease.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022Article
- Lifelong Excess in GH Elicits Sexually Dimorphic Effects on Skeletal Morphology and Bone Mechanical Properties.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022Article
- Review
- Bone matrix quality in a developing high-fat diet mouse model is altered by RAGE deletion.Bone · 2022Article
- Article
- Greater Carboxy-Methyl-Lysine Is Associated With Increased Fracture Risk in Type 2 Diabetes.Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research · 2022Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
It has been a challenge to establish a link between specific advanced glycation end products (AGEs) as causal agents of different pathologies and age-related diseases, primarily because of the lack of suitable in vitro experimental strategies facilitating increased formation of a specific AGE, here carboxymethyllysine (CML), over other AGEs under controlled conditions. CML is of considerable importance to various oxidative stress-related diseases, because in vivo formation of this AGE is connected with cellular oxidative/carbonyl metabolism. The mechanistic implications of CML accumulation in bone remain to be elucidated. To facilitate such studies, we developed a new in vitro strategy that allows preferential generation of CML in bone matrix over other AGEs. Using bone samples from human donors of different age (young, middle-age, and elderly), we show successful in vitro generation of the desired levels of CML and show that they mimic those observed in vivo in several bone disorders. Formation of such physiologically relevant CML levels was achieved by selecting two oxidative/carbonyl stress compounds naturally produced in the human body, glyoxal and glyoxylic acid. Kinetic studies using the two compounds revealed differences not only between their reaction rates but also in the progression and enhanced formation of CML over other AGEs (measured by their collective fluorescence as fluorescent AGEs [fAGEs]) Consequently, through the regulation of reaction time, the levels of CML and fAGEs could be controlled and separated. Given that the developed approach does not fully eliminate the formation of other uncharacterized glycation products, this could be considered as the study limitation. We expect that the concepts of our experimental approach can be used to develop diverse strategies facilitating production of the desired levels of selected AGEs in bone and other tissues, and thus, opens new avenues for investigating the role and mechanistic aspects of specific AGEs, here CML, in bone. © 2021 The Authors.
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