ArticleFrontiers in cellular and infection microbiology2021
Structure-Based Primer Design Minimizes the Risk of PCR Failure Caused by SARS-CoV-2 Mutations.
Article in Frontiers in cellular and infection microbiology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 7 citations in OpenAlex.
- Regulation of protein thermal stability and its potential application in the development of thermo-attenuated vaccines.Engineering microbiology · 2024Article
- The Mutational Landscape of SARS-CoV-2.International journal of molecular sciences · 2023Article
- Computational Analysis Predicts Correlations among Amino Acids in SARS-CoV-2 Proteomes.Biomedicines · 2023Article
- Identification of conserved regions from 230,163 SARS-CoV-2 genomes and their use in diagnostic PCR primer design.Genes & genomics · 2022Article
- Influenza A, Influenza B, and SARS-CoV-2 Similarities and Differences - A Focus on Diagnosis.Frontiers in microbiology · 2022Review
- Sequence similarity of SARS-CoV-2 and humans: Implications for SARS-CoV-2 detection.Frontiers in genetics · 2022Article
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Authors and funding
9 authors at 2 institutions in 1 country.
Funding
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Abstract
The coronavirus disease 2019 (COVID-19) has caused and is still causing tremendous damage to the global economy and human health. Qualitative reverse transcription-PCR (RT-qPCR) is the golden standard for COVID-19 test. However, the SARS-CoV-2 variants may not only make vaccine less effective but also evade RT-qPCR test. Here we suggest an innovative primer design strategy for the RT-qPCR test of SARS-CoV-2. The principle is that the primers should be designed based on both the nucleic acid sequence and the structure of the protein encoded. The three nucleotides closest to the 3' end of the primer should be the codon which encodes the tryptophan in the structure core. Based on this principle, we designed a pair of primers targeting the
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