ArticleCrystal growth & design2021
Droplet-Based Evaporative System for the Estimation of Protein Crystallization Kinetics.
Article in Crystal growth & design, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.
What it found
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Who cites it
5 citing papers in PubMed, 10 citations in OpenAlex.
- Technical Scale in Biomolecular Crystallization.Advances in biochemical engineering/biotechnology · 2026Review
- Image Analysis Methodologies.Advances in biochemical engineering/biotechnology · 2026Review
- An Integrated Experimental and Modeling Approach for Crystallization of Complex Biotherapeutics.Crystal growth & design · 2025Article
- Strategy for Patterning Titania Dendrites by Gas-Solution Interaction at Droplet Surfaces.ACS omega · 2023Article
- Droplet-Based Evaporative System for the Estimation of Protein Crystallization Kinetics.Crystal growth & design · 2021Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Crystallization is a potential cost-effective alternative to chromatography for the purification of biotherapeutic proteins. Crystallization kinetics are required for the design and control of such processes, but only a limited quantity of proteins is available during the initial stage of process development. This article describes the design of a droplet-based evaporative system for the evaluation of candidate crystallization conditions and the estimation of kinetics using only a droplet (on the order of μL) of protein solution. The temperature and humidity of air fed to a flow cell containing the droplet are controlled for evaporation and rehydration of the droplet, which are used for manipulating supersaturation. Dual-angle images of the droplet are taken and analyzed on-line to obtain the droplet volume and crystal sizes. Crystallization kinetics are estimated based on a first-principles process model and experimental data. Tight control of temperature and humidity of the air, fast and accurate image analysis, and accurate estimation of crystallization kinetics are experimentally demonstrated for a model protein lysozyme. The estimated kinetics are suitable for the model-based design and control of protein crystallization processes.
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Registered trials
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