Evidence map›Paper›PMID 34756271›Full record

ArticleAnalytica chimica acta2021

High-resolution imaging and identification of biomolecules using Nano-DESI coupled to ion mobility spectrometry.

Daisy Unsihuay, Ruichuan Yin, Daniela Mesa Sanchez, Manxi Yang, Yingju Li, Xiaofei Sun, Sudhansu K Dey, Julia Laskin

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytica chimica acta, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.

0numbers the graph read from it
0cells of the map it votes in
36citing papers in PubMed
5.8field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

36 citing papers in PubMed, 75 citations in OpenAlex.

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  8. Topological segmentation of mass spectrometry imaging data.Journal of mass spectrometry and advances in the clinical lab · 2025
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 1 country.

Daisy UnsihuayDepartment of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.
Ruichuan YinDepartment of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.
Daniela Mesa SanchezDepartment of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.
Manxi YangDepartment of Chemistry, Purdue University, West Lafayette, IN, 47907, USA.
Yingju LiDivision of Reproductive Sciences, Cincinnati Children's Hospital Medical Centre and Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, 45229, USA.
Xiaofei SunDivision of Reproductive Sciences, Cincinnati Children's Hospital Medical Centre and Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, 45229, USA.
Sudhansu K DeyDivision of Reproductive Sciences, Cincinnati Children's Hospital Medical Centre and Department of Pediatrics, University of Cincinnati College of Medicine, Cincinnati, OH, 45229, USA.
Julia LaskinDepartment of Chemistry, Purdue University, West Lafayette, IN, 47907, USA. Electronic address: jlaskin@purdue.edu.
Purdue University West Lafayette · USCincinnati Children's Hospital Medical Center · US

Funding

Molecular signaling in uterine receptivity to implantationR01HD068524 · NICHD · CINCINNATI CHILDRENS HOSP MED CTR · PI Xiaofei Sun · 2011 to 2026
$5.3M
Endocannabinoid Signaling during Early PregnancyR01HD103475 · NICHD · CINCINNATI CHILDRENS HOSP MED CTR · PI DEY, SUDHANSU K · 2020 to 2024
$2.4M
NICHD NIH HHS R01 HD068524NICHD NIH HHS R01 HD103475
6 · The paper itself

Abstract

Simultaneous spatial localization and structural characterization of molecules in complex biological samples currently represents an analytical challenge for mass spectrometry imaging (MSI) techniques. In this study, we describe a novel experimental platform, which substantially expands the capabilities and enhances the depth of chemical information obtained in high spatial resolution MSI experiments performed using nanospray desorption electrospray ionization (nano-DESI). Specifically, we designed and constructed a portable nano-DESI MSI platform and coupled it with a drift tube ion mobility (IM) spectrometer-mass spectrometer. We demonstrate imaging of drift time-separated ions with a high spatial resolution of better than ∼25 μm using uterine tissues on day 4 of pregnancy in mice. Collision cross-section measurements provide unique molecular descriptors of molecules observed in nano-DESI-IM-MSI necessary for their unambiguous identification by comparison with databases. Meanwhile, isomer-specific imaging reveals variations in the isomeric composition across the tissue. Furthermore, IM separation efficiently eliminates isobaric and isomeric interferences originating from solvent peaks, overlapping isotopic peaks of endogenous molecules extracted from the tissue, and products of in-source fragmentation, which is critical to obtaining accurate concentration gradients in the sample using MSI. The structural information provided by the IM separation substantially expands the molecular specificity of high-resolution MSI necessary for unraveling the complexity of biological systems.

Indexed as

Ion Mobility SpectrometrySpectrometry, Mass, Electrospray IonizationAnimalsDiagnostic Tests, RoutineIonsMiceIonsCollision cross sectionIon mobility spectrometryIsomeric separationLipidomicsMass spectrometry imaging

Identifiers

PMID34756271
PMCPMC8762739
OpenAlexW3199618038

What OpenQuestion holds

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Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.