Evidence map›Paper›PMID 34756066›Full record

ArticleMicrobiology spectrum2021

Spatial and Temporal Constraints on the Composition of Microbial Communities in Subsurface Boreholes of the Edgar Experimental Mine.

Patrick H Thieringer, Alexander S Honeyman, John R Spear

Abstract read
In one paragraph

Article in Microbiology spectrum, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Patrick H ThieringerDepartment of Civil and Environmental Engineering, Colorado School of Minesgrid.254549.b, Golden, Colorado, USA.ORCID 0000-0002-8690-7572
Alexander S HoneymanDepartment of Civil and Environmental Engineering, Colorado School of Minesgrid.254549.b, Golden, Colorado, USA.ORCID 0000-0002-9610-9208
John R SpearDepartment of Civil and Environmental Engineering, Colorado School of Minesgrid.254549.b, Golden, Colorado, USA.ORCID 0000-0002-4664-7438

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The deep biosphere hosts uniquely adapted microorganisms overcoming geochemical extremes at significant depths within the crust of the Earth. Attention is required to understand the near subsurface and its continuity with surface systems, where numerous novel microbial members with unique physiological modifications remain to be identified. This surface-subsurface relationship raises key questions about networking of surface hydrology, geochemistry affecting near-subsurface microbial composition, and resiliency of subsurface ecosystems. Here, we apply molecular and geochemical approaches to determine temporal microbial composition and environmental conditions of filtered borehole fluid from the Edgar Experimental Mine (∼150 m below the surface) in Idaho Springs, CO. Samples were collected over a 4-year collection period from expandable packers deployed to accumulate fluid in previously drilled boreholes located centimeters to meters apart, revealing temporal evolution of borehole microbiology. Meteoric groundwater feeding boreholes demonstrated variable recharge rates likely due to a complex and undefined fracture system within the host rock. 16S rRNA gene analysis determined that unique microbial communities occupy the four boreholes examined. Two boreholes yielded sequences revealing the presence of

Indexed as

MicrobiotaBacteriaBiodiversityDNA, BacterialGeologic SedimentsGroundwaterMiningPhylogenyRNA, Ribosomal, 16SDNA, BacterialRNA, Ribosomal, 16Sfluid-rock interactiongeomicrobiologymicrobial ecologyminingsubsurface microbiology

Identifiers

PMID34756066
PMCPMC8579930

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.