ArticleRedox biology2021
Increased mitochondrial activity upon CatSper channel activation is required for mouse sperm capacitation.
Article in Redox biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The trial behind it
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Who cites it
20 citing papers in PubMed, 47 citations in OpenAlex.
- High-Resolution Imaging Reveals Mitochondrial Protein Imbalance in Sperm of Oligoasthenospermic Men.Molecular reproduction and development · 2026Article
- Targeted metabolomics reveals the impact of glucose and pyruvate on energy metabolism and storage potential of stallion spermatozoa.Metabolomics : Official journal of the Metabolomic Society · 2026Article
- Enhanced CHI3L1 promotes macrophage activation in persistent inflammatory events of ulcerative interstitial cystitis.Frontiers in immunology · 2026Article
- Glucose and pyruvate differentially modulate metabolic and redox dynamics during capacitation to enable fertilization competence in mouse sperm.Reproductive biology and endocrinology : RB&E · 2025Article
- Beyond swimming: emerging parameters for predicting the fertility of mouse spermatozoa.Lab animal · 2025Review
- Bacterial vaginosis toxins impair sperm capacitation and fertilization.Human reproduction (Oxford, England) · 2025Article
- Time and Media-Dependent Modulation of Sperm Capacitation in Red-Rumped Agouti (Dasyprocta leporina Linnaeus, 1758).Molecular reproduction and development · 2025Article
- Effect of Capacitation on Proteomic Profile and Mitochondrial Parameters of Spermatozoa in Bulls.Journal of proteome research · 2025Article
- Mitochondria-Targeted Biomaterials-Regulating Macrophage Polarization Opens New Perspectives for Disease Treatment.International journal of nanomedicine · 2025Review
- Bioenergetics of Rodent Spermatozoa.Methods in molecular biology (Clifton, N.J.) · 2025Article
- Spermatogenic cell-specific type 1 hexokinase (HK1S) is essential for capacitation-associated increase in tyrosine phosphorylation and male fertility in mice.PLoS genetics · 2024Article
- Article
- The Detection of CatSper1 and CatSper3 Expression in Men with Normozoospermia and Asthenoteratozoospermia and Its Association with Sperm Parameters, Fertilization Rate, Embryo Quality.Reproductive sciences (Thousand Oaks, Calif.) · 2024Article
- SLO3: A Conserved Regulator of Sperm Membrane Potential.International journal of molecular sciences · 2023Review
- Ether lipids and a peroxisomal riddle in sperm.Frontiers in cell and developmental biology · 2023Review
- Capacitation induces changes in metabolic pathways supporting motility of epididymal and ejaculated sperm.Frontiers in cell and developmental biology · 2023Article
- Effect of High Viscosity on Energy Metabolism and Kinematics of Spermatozoa from Three Mouse Species Incubated under Capacitating Conditions.International journal of molecular sciences · 2022Article
- Redox regulation by TXNRD3 during epididymal maturation underlies capacitation-associated mitochondrial activity and sperm motility in mice.The Journal of biological chemistry · 2022Article
- Capacitation promotes a shift in energy metabolism in murine sperm.Frontiers in cell and developmental biology · 2022Article
- Mitochondrial metabolism determines the functional status of human sperm and correlates with semen parameters.Frontiers in cell and developmental biology · 2022Article
Corrections and comments
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
Abstract
To fertilize an oocyte, sperm must undergo several biochemical and functional changes known as capacitation. A key event in capacitation is calcium influx through the cation channel of sperm (CatSper). However, the molecular mechanisms of capacitation downstream of this calcium influx are not completely understood. Capacitation is also associated with an increase in mitochondrial oxygen consumption, and several lines of evidence indicate that regulated calcium entry into mitochondria increases the efficiency of oxidative respiration. Thus, we hypothesized that calcium influx through CatSper during capacitation increases mitochondrial calcium concentration and mitochondrial efficiency and thereby contributes to sperm hyperactivation and fertilization capacity. To test this hypothesis, we used high-resolution respirometry to measure mouse sperm mitochondrial activity. We also measured mitochondrial membrane potential, ATP/ADP exchange during capacitation, and mitochondrial calcium concentration in sperm from wild-type and CatSper knockout mice. We show that the increase in mitochondrial activity in capacitated wild-type sperm parallels the increase in mitochondrial calcium concentration. This effect is blunted in sperm from CatSper knockout mice. Importantly, these mechanisms are needed for optimal hyperactivation and fertilization in wild-type mice, as confirmed by using mitochondrial inhibitors. Thus, we describe a novel mechanism of sperm capacitation. This work contributes to our understanding of the role of mitochondria in sperm physiology and opens the possibility of new molecular targets for fertility treatments and male contraception.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.