ArticleMethods in cell biology2021
Evaluating functional ligand-GPCR interactions in cell-based assays.
Article in Methods in cell biology, 2021. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
What it found
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Who cites it
7 citing papers in PubMed.
- A brain-gut excitatory peptide/CCHamide homolog regulates satiation and motivational state transitions in the Aplysia feeding circuit.The Journal of biological chemistry · 2026Article
- The Mechanism of LTXInternational journal of molecular sciences · 2025Article
- Identification of a G Protein-Coupled Receptor for Buccalin-Type Peptides in the MolluskACS omega · 2025Article
- Synaptic and intrinsic plasticity mediated by CCK-type signaling coordinates behavioral changes during motivational state shifts.Cell reports · 2025Article
- Affinity-Driven Aryl Diazonium Labeling of Peptide Receptors on Living Cells.Journal of the American Chemical Society · 2024Article
- Endogenous l- to d-amino acid residue isomerization modulates selectivity between distinct neuropeptide receptor family members.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Characterization of anFrontiers in pharmacology · 2023Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
G protein-coupled receptors (GPCRs) are a family of transmembrane proteins that act as major mediators of cellular signaling, and are the primary targets for a large portion of clinical therapeutics. Despite their critical role in biology and medicine, a large number of GPCRs are poorly understood, lacking validated ligands or potent synthetic modulators. Ligand-induced GPCR activation can be measured in cell-based assays to test hypotheses about ligand-receptor interactions or to evaluate efficacy of synthetic agonists or antagonists. However, the techniques necessary to develop and implement a cell-based assay to study a given receptor of interest are not commonplace in all laboratories. This chapter outlines methods to develop a cell-based assay to evaluate agonist-induced activation for a GPCR of interest, which can be useful to evaluate the effectiveness of predicted ligands. Examples of sample preparation protocols and data analysis are provided to help researchers from interdisciplinary fields, especially those in fields with relatively little molecular biology or cell culture experience.
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Registered trials
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